Giorgio Arrigoni
Giorgio Arrigoni
affiliation: Università di Padova
research area(s): Chemical Biology
Course: Biosciences and Biotechnologies
University/Istitution: Università di Padova
Academic Positions

2008-present Assistant Professor, Department of Biomedical Sciences, University of Padova, Italy

2007-2008 Research Engineer at the Department of Immunotechnology/Protein Technology, University of Lund, Sweden

Education and Training

2003-2006 Post-doctoral fellow at the Department of Immunotechnology/Protein Technology, University of Lund, Sweden and at the Department of Biological Chemistry, University of Padova, Italy

2000-2003 Ph.D. in Biochemistry and Biophysics, University of Padova, Italy

2000 Chemist qualifying examination

1999 Degree in Chemistry, University of Padova, Italy


2003- Fellowship granted by the foundation "Ing. Aldo Gini", Padova (Italy) for a period of 8 months to spend at Lund University (Sweden)

2004- Fellowship granted by the foundation "Blanceflor Boncompagni-Ludovisi" (Stockholm, Sweden) for a period of 12 months.

2005- Short Term Fellowship granted by EMBO (European Molecular Biology Organization).

2006- Fellowship granted by the foundation "Blanceflor Boncompagni-Ludovisi" (Stockholm, Sweden) for a period of 12 months.

2006- Travel Grant granted by the Kungliga Fysiografiska Sällskapet (Royal Physiographic Society), Lund, Sweden

2007- Travel Grant granted by the Kungliga Fysiografiska Sällskapet (Royal Physiographic Society), Lund, Sweden
Dr. Arrigoni started his scientific activity in the field of protein chemistry, focusing his interests on peptide synthesis and structural and biochemical studies on peptides and proteins, with special reference to the CK2-dependent protein phosphorylation.

During his Ph.D. he started a collaboration with the Department of Protein Technology, Lund University (Sweden) where, under the supervision of Prof. Peter James, he specialized in Proteomics and mass spectrometry methodologies with special reference to the study of protein phosphorylation and protein-protein interaction.

During his Post-doc he was strongly involved in the development of chemicals for derivatization of proteins and in the development of chemical and instrumental methods for the qualitative and quantitative analysis of proteomes by mass spectrometry. The study of protein phosphorylation, especially CK2-mediated, is always a dominant part of his research activity.

Dr. Arrigoni is currently on the list of reviewers for the following journals: Proteomics, Proteomics Clinical Applications, Journal of Proteome Research, Analytical and Bioanalytical Chemistry.
Tolin S, Arrigoni G, Moscatiello R, Masi A, Navazio L, Sablok G, Squartini A. (2012) Quantitative Analysis of Naringenin-inducible Proteome in Rhizobium leguminosarum by Isobaric Tagging and Mass Spectrometry. Submitted to

Tolin S, Arrigoni G, Zechmann B, Trentin A, Jovanović SV, Masi A (2012) Biochemical investigations and proteomic profiling in ggt1-mutant leaves reveal a role for the gamma-glutamyl cycle in the plant’s adaptation to the environment.
Submitted to Proteomics.

Salvi M, Trashi E, Cozza G, Franchin C, Arrigoni G, Pinna LA (2012) Investigation on PLK2 and PLK3 substrate recognition. Biochim Biophys Acta; Dec;1824(12):1366-73

Dosselli R, Millioni R, Puricelli L, Tessari P, Arrigoni G, Franchin C, Segalla A, Teardo E, Reddi E. (2012) Molecular Targets of Antimicrobial Photodynamic Therapy Identified by a Proteomic Approach. JOURNAL OF PROTEOMICS,Sep 20. pii:S1874-3919(12)00651-3

Tolin S, Pasini G, Simonato B, Mainente F, Arrigoni G (2012). Analysis of Commercial Wines by LC-MS/MS Reveals the Presence of Residual Milk and Egg White Allergens FOOD CONTROL 321- 326, 28

Tolin S, Pasini G, Curioni A, Arrigoni G, Masi A, Mainente F, Simonato B (2012). Mass spectrometry detection of egg proteins in red wines treated with egg white FOOD CONTROL 87- 94, 23

Millioni R, Tolin S, Fadini GP, Falda M, van Breukelen B, Tessari P, Arrigoni G (2012). High confidence and sensitivity four-dimensional fractionation for human plasma proteome analysis. AMINO ACIDS, in Press

Millioni R; Tolin S; Puricelli L; Sbrignadello S; Fadini GP; Tessari P; Arrigoni G. (2011). High Abundance Proteins Depletion vs Low Abundance Proteins Enrichment: Comparison of Methods to Reduce the Plasma Proteome Complexity PLOS ONE, e19603- 6

R. Ballardini, M. Benevento, G. Arrigoni, L. Pattini, A. Roda (2011). MassUntangler: A novel alignment tool for label-free liquid chromatography/mass spectrometry proteomic data JOURNAL OF CHROMATOGRAPHY A 8859-8868, 1218

Bertacco E; Millioni R; Arrigoni G; Faggini E; Iop L.; Puato M; Pinna LA; Tessari P; Pauletto P; Rattazzi M. (2010). Proteomic analysis of clonal interstitial aortic valve cells acquiring a pro-calcific profile. JOURNAL OF PROTEOME
RESEARCH 5913- 5921, 9

Tibaldi E; Arrigoni G; Martinez HM; Inagaki K; Shimasaki S; Pinna L.A. (2010). Golgi apparatus casein kinase phosphorylates bioactive Ser-6 of bone morphogenetic protein 15 and growth and differentiation factor 9 FEBS LETTERS 801-805, 584

Bendz M; Moller MC; Arrigoni G.; Wahlander A; Stella R; Cappadona S; Levander F; Hederstedt L; James P (2009). Quantification of Membrane Proteins Using Nonspecific Protease Digestions JOURNAL OF PROTEOME RESEARCH 5666-5673, 8

Cenni V; Bertacchini J; Beretti F; Lattanzi G; Bavelloni A; Riccio M; Ruzzene M; O. Marin; Arrigoni G; Parnaik V; Wehnert M; Maraldi NM; De Pol A; Cocco L; Marmiroli S (2008). Lamin A Ser404 Is a Nuclear Target of Akt Phosphorylation in C2C12 Cell JOURNAL OF PROTEOME RESEARCH 4727-4735, 7 (11)

Arrigoni G, Pagano MA, Sarno S, Cesaro L, James P, Pinna LA (2008). Mass spectrometry analysis of a protein kinase CK2 beta subunit interactome isolated from mouse brain by affinity chromatography JOURNAL OF PROTEOME RESEARCH 990-1000, 7

Pagano MA, Arrigoni G, Marin O, Sarno S, Meggio F, Treharne KJ, Mehta A, Pinna LA (2008). Modulation of protein kinase CK2 activity by fragments of CFTR encompassing F508 may reflect functional links with cystic fibrosis pathogenesis BIOCHEMISTRY 7925-7936, 47

Wahlander A; Arrigoni G; Warell K; Levander F; Palmgren R; Maloisel Jl; Busson P; James P (2007) Development of reagents for differential protein quantitation by subtractive parent (precursor) ion scanning JOURNAL OF PROTEOME RESEARCH 1101-1113, 6

G. Arrigoni; Fernandez C; Holm C; Scigelova M; James P (2006). Comparison of MS/MS methods for protein identification from 2D-PAGE JOURNAL OF PROTEOME RESEARCH 2294-2300, 5

Arrigoni G.; Resjo S.; Levander F.; Nilsson R.; Degerman E.; Quadroni M.; Pinna L.A.; James P. (2006). Chemical derivatization of phospho-serine and -threonine peptides to increase MALDI sensitivity and selectivity of MS/MS analysis PROTEOMICS 757-766, 6

Berggard T; Arrigoni G; Olsson O; Fex M; Linse S; James P (2006). 140 Mouse brain proteins identified by Ca2+-calmodulin affinity chromatography and tandem mass spectrometry JOURNAL OF PROTEOME RESEARCH 669-687, 5

Tibaldi E; Arrigoni G; Brunati AM; James P; Pinna LA (2006). Analysis of a sub-proteome which co-purifies with and is phosphorylated by the Golgi casein kinase. CELLULAR AND MOLECULAR LIFE SCIENCES 378-389, 63(3)

Di Maira G; Salvi M; Arrigoni G; Marin O; Sarno S; Brustolon F; Pinna LA; M. Ruzzene (2005). Protein kinase CK2 phosphorylates and upregulates Akt/PKB CELL DEATH AND DIFFERENTIATION 668-677, 12
Project Title:
High-throughput Measurements of Cellulat and Tissue Protein Turnover
The project requires the application of state-of-the-art proteomics techniques to study the protein turnover rate.
Changes in protein turnover, which are not measured in classic Proteomics studies, could be the key to solve discrepancies observed between transcriptomics and proteomics data. Therefore, measurements of individual protein synthesis and degradation rates can prove to be a highly innovative and potentially powerful approach, to fully account for transduction and translation data.