Livia Bianchi
Livia Bianchi
e-mail:
affiliation: Università di Pavia
research area(s): Cancer Biology, Experimental Medicine
Course: Genetics, Molecular and Cellular Biology
University/Istitution: Università di Pavia
ACCADEMIC POSITION

Associate Professor of General Pathology (MED04), Dip of Experimental Medicine, Faculty of Sciences, University of Pavia, Via Ferrata 1, 27100 Pavia, Italy.
Fax (39) 0382-986893
e-mail liviabianchi@unipv.it

PLACE AND DATE OF BIRTH : Genova 5 february 1951

PROFESSIONAL EXPERIENCE

Since 2000 Associate Professor of General Pathology (MED04) at the Faculty of Sciences
1992-2000 Associate Professor of General Pathology (MED04) at the Faculty of Medicine and Surgey
1986-1992 " Tecnico laureato" at the Institute of General Pathology, University of Pavia, Italy.
1982-1986 Research fellowship at the Institute of General Pathology, University of Pavia, Italy. 1980-1982 Research fellowship at the Institute of Biochemistry and Genetics, University of Louvain, Belgium.
1975-1980 Research fellowship at the Institute of Genetics, University of Parma, Italy.
1975 Graduated in Biological Sciences, University of Bologna, Italy

GRANTS

2000-2003 European Project:Anthocyanin bioactivities QLK1-1999-00124
2004-2006 European Project :Highly Selective and Environmentally Friendly fruit extraction using supercritical fluids technology COOP-CT-2004- 512550
2005-2007 PRIN Project :Proliferazione estrogeno-dipendente e indipendente:studio del meccanismo d"azione del resveratrolo e di alcuni suoi derivati di sintesi
2008-2010 PRIN Project: Interazioni funzionali tra recettori di membrana e nucleari nella trasduzione di segnali estrogenici e di fattori di crescita nei tumori ormono-responsivi
The main research has focused on biological activity of natural or synthetic derived compounds with a potential role in the prevention of human pathological processes, such as cancer. Our work have clarified the mechanism of action, through the identification of protein targets, of the antiproliferative and/or antioxidant effect of some natural agents, such as beta-carotene, anthocyanins and, more recently, stilbenes.The antiproliferative effect of resveratrol, a phytoalexin present in red wine, has been demonstrated in normal and in malignant fibroblast cultures. The synthesis of some resveratrol derivatives in which all or each single hydroxylic function were selectively substituted with methyl groups has allowed to correlate the molecular structure with the biological activity. In particular a new compound 4,4" dihydroxystilbene has shown in human fibroblasts and in breast cancer cell an higher antiproliferative and in human endhothelial cells an higher vasoprotective effect if compared to resveratrol. The biological effects of two anthocyanins (delphinidine and cyanidine) present in blackcurrant were studied. It has been demonstrated that these two compounds are protective against the redox state and oxidative DNA damage induced by tert-butyl-hydroperoxide both in normal and tumor cell lines. Moreover, an antiproliferative effect of cyanidine and delphinidine in normal human fibroblast was observed; only delphinidine showed pro-apoptotic effect in tumor cell lines. Finally the vasoprotective activity of the two compounds has been demonstrated in human endhothelial cells.Finally it has been demonstrated an antioxidant and antiproliferative effect induced by grape extracts. obtained using supercritical fluids technology. The aim of our studies is now to evaluate the biological effects detected in colture cells in animal models
C. SCOTTI, L. IAMELE, A. ALESSANDRINI, V. VANNINI, O. CAZZALINI, M.C. LAZZE", R. MELLI, M. SAVIO, R. PIZZALA, L.A.STIVALA, S. BIGLIERI, A. TOMASI, L. BIANCHI.
Lack of molecular relationships between lipid peroxidation and mitochondrial DNA single strand breaks in isolated hepatocytes and mitochondria.
Mitochondrion, 2, 361-373, 2003.

MC LAZZE", R PIZZALA., M SAVIO., LA STIVALA, E PROSPERI, BIANCHI L.
Anthocyanins protect against DNA damage induced by tert-butyl-hydroperoxide in rat smooth muscle and hepatoma cells
Mutat Res., 535, 103-15, 2003

O. CAZZALINI, P. PERUCCA, F. RIVA, L.A. STIVALA, L. BIANCHI, V. VANNINI, B. DUCOMMAN, E. PROSPERI.
p21CDKN1A does not interfere with loading of PCNA at DNA replication sites, but inhibits subsequent binding of DNA polymerase delta at the G1/S phase transition.
Cell Cycle, 2(6), 596-603, 2003

M.C. LAZZE", M. SAVIO, PIZZALA R., O. CAZZALINI, P. PERUCCA, A.I. SCOVASSI, L.A. STIVALA, BIANCHI L.
Anthocyanins induce cell cycle perturbations and apoptosis in different human cell lines.
Carcinogenesis, 25(8), 1427-33, 2004.

O. CAZZALINI, P. PERUCCA, F. VALSECCHI, L.A. STIVALA, L. BIANCHI, V. VANNINI, E. PROSPERI.
Intracellular localization of the cyclin-dependent kinase inhibitor p211DKN1A-GFP fusion protein during cell cycle arrest.
Histochem Cell Biol., 121(5), 377-81, 2004.

LAZZE"M.C., PIZZALA R., PERRUCA P. CAZZALINI O., SAVIO M., , FORTI L., VANNINI V. BIANCHI L., Anthocyanidins decrease endothelin-1 production and increase endothelial nitric oxide synthase in human endothelial cells, Molecular Nutrition and Food Research, 50 (1), pp. 44-51. 2006,

O. CAZZALINI, P. PERUCCA, M. SAVIO, D. NECCHI, L. BIANCHI, L.A. STIVALA, B. DUCOMMUN, A.I. SCOVASSI, AND E. PROSPERI
Interaction of p21CDKN1A with PCNA regulates the histone acetyltransferase activity of p300 in nucleotide excision repair
Nucleic Acids Res., 36 (5), 1713-22, 2008.

M. SAVIO, T. COPPA, L. BIANCHI, V. VANNINI, G. MAGA, L. FORTI, O. CAZZALINI, M.C. LAZZE", P. PERUCCA, E. PROSPERI, L.A. STIVALA
The resveratrol analogue 4,4'-dihydroxy-trans-stilbene inhibits cell proliferation with higher efficiency but different mechanism from resveratrol.
Int J Biochem Cell Biol., 41, 2493-2502, 2009.
M.C LAZZE, R.PIZZALA` F. Jrì GUTTIERREZ PECHARROMA,P. G. GARNICA, J. M. ANTOLIN RODRIGUEZodr ",N. FABRIS, L BIANCHI
Grape Waste Extract Obtained by Supercritical Fluid Extraction Contains Bioactive Antioxidant Molecules and Induces Antiproliferative Effects in Human Colon Adenocarcinoma Cells
J Med Food 12 (3, 561"568) 2009

COPPA T, LAZZE’ MC, CAZZALINI O, PERRUCA P, PIZZALA R, BIANCHI L; STIVALA LA, FORTI L, MACCARIO C, VANNINI V, SAVIO M.
Structure- Activity Relationship of Resveratrol and Its analogue 4,4’- Dihydroxy-Trans-Stilbene Toward the Endothelin Axis in Human Endothelial Cells.
J Med Food 14(10), 1173-1180 2011

The resveratrol analogue 4,4'−dihydroxy−trans−stilbene suppresses transformation in normal mouse fibroblasts and inhibits proliferation and invasion of human breast cancer cells
Cristina Maccario, Monica Savio, Daniela Ferraro, Livia Bianchi, Roberto Pizzala, Luca Pretali, Luca Forti, Lucia Anna Stivala.
Carcinogenesis, 2012, DOI: 10.1093/carcin/bgs244
No projects are available to students for the current accademic year.