Claudia Binda
Claudia Binda
affiliation: Università di Pavia
research area(s): Molecular Biology, Chemical Biology
Course: Genetics, Molecular and Cellular Biology
University/Istitution: Università di Pavia
Claudia Binda
born in Varese (Italy) in 1973, married, mother of Michela
Università di Pavia
Dip. Biologia e Biotecnologie
via Ferrata 1 – Pavia
Tel. 0382-985527, Fax 0382-528496


1997 Degree in Biological Sciences conferred at the University of Pavia.
1997-2000 PhD at the University of Pavia. Area of research: structural biology of polyamine oxidase and glutamate synthase (mentor: Prof. Andrea Mattevi).

Professional experience

2000-2001 Post-doc supported by NIH (USA) for structural investigations of human monoamine oxidase, a drug target for the treatment of neurodegenerative diseases (University of Pavia).
2002, March-April Biochemical studies on human monoamine oxidase in the Laboratory of Biochemistry of Prof. Dale Edmondson (Emory University, Atlanta, USA).
2002-2004 Post-doc supported by a grant from Pfizer. Area of research: characterization of a transcriptional co-repressor complex that controls neuron differentiation (University of Pavia).
2005-2007 Post-doc “Alunno speciale MINTAS” supported by Collegio Ghislieri for structural and biochemical studies on monoamine oxidases and lysine-specific histone demethylase (University of Pavia).
2008-2015 Permanent position as Assistant Professor at the University of Pavia, Dept. Biology and Biotechnology.
2015-present Permanent position as Associate Professor at the University of Pavia, Dept. Biology and Biotechnology.

Teaching and other appointments

2009-present teacher for various courses (Molecular Biology, Laboratory of Molecular Biology, Methods for Protein Engineering) in both bachelor degree and master degree programs.
2008-present member of the PhD program in Genetics, Molecular and Cell Biology.

2013-2019 member of the Orientation Center (COR) of the University of Pavia to assist students in their academic studies in the area of Biotechnology.
2013-2017 member of the teaching joint committee of the Department of Biology and Biotechnology.
2018-2019 Coordinator of the master degree in Advanced Biotechnology.
2019-present Chair of the Teaching Board in Biotechnology.


1998 Prize for the best poster at the conference “PROTEINE 1998”, Genova (Italy).
2002 Prize “Il nuovo a Pavia” from “Camera di Commercio” of Pavia for the work on human monoamine oxidase.
2007 Prize “Enrico Gavuzzo 2007” from “Istituto di Cristallografia del CNR” (Roma) for young researchers working in the field of drug design.
2012 Prize “BioEconomy Rome 2012” from Consorzio CNCCS (Roma) for research in the field of poverty, rare and neglected diseases.
The research activity of Claudia Binda is carried out in the Structural Biology group of the Department of Genetics and Microbiology at the University of Pavia ( Her main interest is the study of proteins and protein complexes of medical relevance and for industrial applications. In particular, she is focused on a class of enzymes which use FAD as cofactor to catalyze biochemical reactions (such as amine oxidation) in many different cellular contexts, ranging from neurotransmitters metabolism to gene expression regulation to mycobacteria cell wall biosynthetic pathways. The aim of this work is to characterize these proteins at molecular level to unravel the underlying cellular mechanisms and macromolecular interactions and to use this knowledge to provide clues for the development of pharmacological treatment of human diseases. The methodological approach involves gene cloning, E. coli and P. pastoris recombinant protein expression, biochemical characterization of enzymes by spectrophotometric and fluorimetric assays, crystallization and three-dimensional structure determination by X-ray crystallography. The laboratory can count also on established collaborations with cell biology, microbiology, organic chemistry and biochemistry groups both within Italy and in other countries.
List of selected publications (total = 63, Scopus h-index = 39)

1. Binda, C., Coda, A., Angelini, R., Federico, R., Ascenzi, P., Mattevi, A. (1999). A 30 Å long U-shaped catalytic tunnel in the crystal structure of polyamine oxidase. Structure, 7, 265-276.
2. Binda, C., Bossi, R.T., Wakatsuki, S., Arzt, S., Coda, A., Curti, B., Vanoni, M.A., Mattevi, A. (2000). Cross-talk and ammonia channeling between active centers in the unexpected domain arrangement of glutamate synthase . Structure 8, 1299-1308.
3. Binda, C., Newton-Vinson, P., Hubalek, F., Edmondson, D.E., Mattevi, A. (2002). Structure of human monoamine oxidase B, a drug target for the treatment of neurological disorders. Nat. Struct. Biol. 9, 22-26.
4. Binda, C., Li, M., Hubalek, F., Restelli, N., Edmondson, D. E., Mattevi, A. (2003). Insights into the mode of inhibition of human mitochondrial monoamine oxidase B from high-resolution crystal structures. Proc. Natl. Acad. Sci. USA, 100, 9750-9755.
5. Binda, C., Hubalek, F., Li, M., Herzig, Y., Sterling, J., Edmondson, D.E., Mattevi, A. (2004). Crystal Structures of MAO B in Complex with Four Inhibitors of the N-Propargylaminoindan Class. J. Med. Chem. 47, 1767-1774.
6. Binda, C., Hubalek, F, Li, M., Herzig, Y., Sterling, J., Edmondson, D.E., Mattevi, A. (2005). Binding of rasagiline-related inhibitors to human monoamine oxidases: a kinetic and crystallographic analysis. J. Med. Chem. 48, 8148-8154.
7. Binda, C., Hubalek, F., Li, M., Castagnoli, N., Edmondson, D.E., Mattevi, A. (2006). Structure of the human mitochondrial monoamine oxidase B: new chemical implications for neuroprotectant drug design. Neurology 67, S5-7.
8. Binda, C., Wang, J., Pisani, L., Caccia, C., Carotti, A., Salvati, P., Edmondson, D.E., Mattevi, A. (2007). Structures of Human Monoamine Oxidase B Complexes with Selective Noncovalent Inhibitors: Safinamide and Coumarin Analogs. J. Med. Chem. 50, 5848-5852.
9. Binda, C., Wang, J., Li, M., Hubalek, F., Mattevi, A., Edmondson, D.E. (2008). Structural and mechanistic studies of arylalkylhydrazine inhibition of human monoamine oxidases A and B. Biochemistry, 47, 5616-5625.
10. Binda, C., Valente, S., Romanenghi, M., Pilotto, S., Cirilli, R., Karytinos, A., Ciossani, G., Botrugno, O.A., Forneris, F., Tardugno, M., Edmondson, D.E., Minucci, S., Mattevi, A., Mai, A. (2010) Biochemical, Structural, and Biological Evaluation of Tranylcypromine Derivatives as Inhibitors of Histone Demethylases LSD1 and LSD2. J. Am. Chem. Soc. 132, 6827-6833.
11. Binda C., Aldeco, M., Mattevi A., Edmondson D.E. (2011) Interactions of Monoamine Oxidases with the Antiepileptic Drug Zonisamide: Specificity of Inhibition and Structure of the Human Monoamine Oxidase B Complex. J. Med. Chem., 54, 909-912.
12. Binda, C., Mattevi, A., Edmondson, D.E. (2011) Structural properties of human monoamine oxidases A and B. Int. Rev. Neurobiol., 100, 1-11.
13. Binda, C., Milczek, E.M., Bonivento, D., Wang, J., Mattevi, A., Edmondson, D.E. (2011) Lights and Shadows on Monoamine Oxidase Inhibition in Neuroprotective Pharmacological Therapies. Curr. Top. Med. Chem., 11, 2788-2796.
14. Binda, C., Aldeco, M., Geldenhuys, W.J., Tortorici, M., Mattevi, A., Edmondson, D.E. (2012) Molecular Insights into Human Monoamine Oxidase B Inhibition by the Glitazone Antidiabetes Drugs. ACS Med. Chem. Lett. 3, 39-42.
15. Neres, J., Pojer, F., Molteni, E., Chiarelli, L.R. Dhar, N., Boy-Röttger, S., Buroni, S., Fullam, E. Degiacomi, G., Lucarelli, A., Read, R.J., Zanoni, G., Edmondson, D.E., De Rossi, E., Pasca, M., McKinney, J.D., Dyson, P.J., Riccardi, G., Mattevi, A., Cole, S.T., Binda, C. (2012) Structural basis for benzothiazinone-mediated killing of Mycobacterium tuberculosis. Sci. Transl. Med. 4, 150ra121.
16. Binda, C.*, Robinson, R.M.*, Martin Del Campo, J.S., Keul, N.D., Rodriguez, P.J., Robinson, H.H., Mattevi, A., Sobrado, P. (2015) An Unprecedented NADPH Domain Conformation in Lysine Monooxygenase NbtG Provides Insights Into Uncoupling of Oxygen Consumption From Substrate Hydroxylation. J. Biol. Chem., 290, 12676-12688. *These two authors contributed equally to this work.
17. Edmondson, D.E., Binda, C. (2018) Monoamine Oxidases. In “Membrane Protein Complexes: Structure and Function” (Springer Nature Singapore Pte Ltd. 2018, J. R. Harris, E. J. Boekema eds.), Subcell. Biochem., 87, 117-139.
18. Reis, J., Manzella, N., Cagide, F., Mialet-Perez, J., Uriarte, E., Parini, A., Borges, F., Binda, C. (2018) Tight-Binding Inhibition of Human Monoamine Oxidase B by Chromone Analogs: A Kinetic, Crystallographic, and Biological Analysis. J. Med. Chem., 61, 4203-4212.
19. Iacovino, L.G., Magnani, F., Binda, C. (2018) The structure of monoamine oxidases: past, present, and future. J. Neural. Transm., 125, 1567-1579.
Project Title:
Structural and biochemical studies on the drug target enzymes Monoamine Oxidases