Maria Grazia Bottone
Maria Grazia Bottone
affiliation: Università di Pavia
research area(s): Cell Biology, Neuroscience
Course: Genetics, Molecular and Cellular Biology
University/Istitution: Università di Pavia
Born in Voghera, 1965;
From 2002, to present, University Researcher (SSD BIO/06), Faculty of Sciences, University of Pavia ;
From 2004, to present, Associate member of the Institute of Molecular Genetics of the National Research Council, in Pavia;
Currently, she is teacher of the courses: “ Human Neuroanatomy" and “Neurogenesis and Comparative Neuromorphology" (Form 2) for specialized Academic Degree in "Neurobiology”– University of Pavia;
It was teacher of the courses: “Cellular and Morphological laboratory techniques” for specialized Academic Degree in Sciences of Nature – University of Pavia (2004-2010);
To present, is Associated Investigator of the Research Unit Program Project Prin 2009 “Neurotoxicity evaluation "in vitro" and "in vivo" of new platinum compounds”;
Since 1994 he is responsible for organizing and conducting regular courses in cytometric and microscopic techniques for learning and upgrading of teachers and technicians who work in research and analysis in the biomedical field;
Technician at the Cell Biology Laboratory, Dipartimento di Biologia Animale, University of Pavia (November 1998 to November 2002);
Assistant Technician at the Flow Cytofluorometry Laboratory of the Centro Grandi Strumenti, University of Pavia, (1998);
He held postdoctoral fellowships at the Laboratory of Histology and Embryology, Department of Animal Biology, University of Pavia (1996-1998);
Postgraduate Research Grant,(Hystology and Embryology Laboratory, Animal Biology Department,University of Pavia (1994 to 1996);
Lab Technician in the Flow Cytofluorometry Laboratory of the Centro Grandi Strumenti, University of Pavia (1993-1994);
PhD in Cytochemistry and Cytomorphology- University of Pavia (1993);
Degree in Biological Science at the University of Pavia (1988).
In collaboration with several University Research Institutes and with Research Centres in Italy and abroad (San Raffaele Hospital Milan, Italy; Pharmacological Sciences Institute and General Pathology Institute of the University of Milan, Molecular Genetics Institute of the CNR Pavia; Research Institute IRCCS San Matteo Pavia, Physiology institute of the Academy of Sciences of the Czech Republic) concerns the study of cytokynetic parameters in different cell systems in culture, under drug treatment or physical stress, and the the study of the mechanisms inducing apoptotic cell death with special reference to the apoptogenic action of photosensitizing molecules (Projects Prin 2002-2003 and 2005-2007). More recently, in collaboration with University of Salento, Italy, the research activitiy has been focussed on the study in vivo and in vitro of neurotoxic effects of cisplatin, and of cisplatin compounds. Particulary, is studying the processes of apoptosis and autophagy in neural cultured cells (Project Prin 2009, Project Fondazione Banca del Monte di Lombardia).
1. CROCE A.C., FASANI E., BOTTONE M.G., DE SIMONE U., SANTIN G., PELLICCIARI C., BOTTIROLI G.(2011) Hypocrellin-B acetate as a fluorogenic substrate for enzyme-assisted cell photosensitization. Photochem Photobiol Sci.
2. SANTIN G., PICCOLINI V.M., VENERONI P., BARNI S., BERNOCCHI G., BOTTONE M.G.(2011) Different patterns of apoptosis in response to cisplatin in B50 neuroblastoma rat cells. Histol Histopathol. 26(7),831-842.
3. BERNOCCHI G., BOTTONE M.G., PICCOLINI V.M., DAL BO V., SANTIN G., DE PASCALI S.A., MIGONI D., FANIZZI, F.P.(2011) Developing central nervous systemand vulnerability to platinum compounds. Chemotherapy Research and Practice, 1-14.
4. CERRI S., PICCOLINI V.M., SANTIN G., BOTTONE M.G., DE PASCALI S.A., MIGONI D., IADAROLA P., FANIZZI F.P., BERNOCCHI G.(2011) The developmental neurotoxicity study of platinum compounds. Effects of cisplatin versus a novel Pt(II) complex on rat cerebellum. Neurotoxicol Teratol. vol. 233, 273-81.
5. BOTTONE M.G., GIANSANTI V., VENERONI P., SCOVASSI A. I., BERNOCCHI G., PELLICCIARI C. (2010) The Apoptosis-Inducing Factor (AIF) Moves from Mitochondria to the Nucleus and Back to the Cytoplasm, during Apoptosis. In: Oliver L. Svensson. Mitochondria: Structure, Functions and Dysfunctions. Nova Science Publishers, Inc
6. BALESTRAZZI A., LOCATO V., BOTTONE M.G., DE GARA L., BIGGIOGERA M., PELLICCIARI C., BOTTI S., DI GESÙ D., DONÀ M., CARBONERA D.(2010) Response to UV-C radiation in topo I-deficient carrot cells with low ascorbate levels. J Exp Bot. vol. 61(2), 575-85.
7. BOTTONE M.G., SOLDANI C., FRASCHINI A., CROCE A.C., BOTTIROLI G., CAMBONI T., SCOVASSI A.I., PELLICCIARI C. (2009) Enzyme-assisted photosensitization activates different apoptotic pathways in Rose bengal acetate treated He-La cells. HISTOCHEMISTRY AND CELL BIOLOGY, vol. 131, 391-399.
8. CERRI S., BOTTIROLI G., BOTTONE M.G., BARNI S., BERNOCCHI G. (2009) Cell proliferation and death in the brain of active and hibernating frogs. JOURNAL OF ANATOMY, vol. 215, 124-131.
9. SCOVASSI A.I., SOLDANI C., VENERONI P., BOTTONE M.G., PELLICCIARI C. (2009) Changes of mitochondria and relocation of the Apoptosis Inducing Factor (AIF) during apoptosis. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, vol. 1171, 12-17.
10. SCOVASSI A.I., BOTTONE M.G., BIGGIOGERA M., PELLICCIARI C. (2008) Dynamic relocation of nuclear proteins during the execution phase of apoptosis. BIOCHEMICAL PHARMACOLOGY, vol. 76, 1440-1450.
11. BOTTONE M.G., SOLDANI C, VENERONI P, AVELLA D, PISU M, BERNOCCHI G (2008) Cell proliferation, apoptosis and mitochondrial damage in rat B50 neuronal cells after cisplatin treatment. CELL PROLIFERATION, vol. 41, 506-520.
12. BIGGIOGERA M., CISTERNA B., BOTTONE M.G., SOLDANI C., PELLICCIARI C. E. (2007) Nuclear RNP and Nucleolar-Associated Proteins during Apoptosis: a Politically Correct Form of Segregation?. DYNAMIC CELL BIOLOGY, vol. 1, 65-71.
13. BOTTONE M.G., SOLDANI C, A. FRASCHINI, ALPINI C, CROCE A.C, BOTTIROLI G, PELLICCIARI C (2007) Enzyme-assisted photosensitization with Rose Bengal acetate induces structural and functional alteration of mitochondria in HeLa cells. HISTOCHEMISTRY AND CELL BIOLOGY, vol. 127, 263-271.
14. BOTTONE M.G., SOLDANI C., FRASCHINI A., ALPINI C., CROCE A.C., BOTTIROLI G., PELLICCIARI C. (2007) Enzyme-assisted photosensitization with rose Bengal acetate induces structural and functional alteration of mitochondria in HeLa cells. HISTOCHEMISTRY AND CELL BIOLOGY, vol. 127, 263-271.
15. SOLDANI C, BOTTONE M.G., FRASCHINI A, CROCE A.C. BOTTIROLI G, SCOVASSI A. I, PELLICCIARI C. (2007) Apoptosis as a Consequence of Multiple Organelle Damage in Cultured Tumor Cells after Photosensitization with Rose Bengal Acetate. In: ALBINA W. TAYLOR Ed.. Cell Apoptosis and Cancer. Nova Science Publishers.
16. SOLDANI C., CROCE A.C., BOTTONE M.G., FRASCHINI A., BIGGIOGERA M., BOTTIROLI G., PELLICCIARI C. (2007) Apoptosis in tumor cells photosensitized wity Rose Bengal Acetate is induced by multiple organelle photodamage. HISTOCHEMISTRY AND CELL BIOLOGY, vol. 128, 485-495.
17. KRAJCI D., MARES V., LISA V., BOTTONE M.G., PELLICCIARI C. (2006) Intranuclear microtubules are hallmarks of an unusual form of cell death in cisplatin-treated C6 glioma cells. HISTOCHEMISTRY AND CELL BIOLOGY, vol. 125, 183-191.
18. SOLDANI C., BOTTONE M.G., PELLICCIARI C., SCOVASSI A.I (2006) Nucleolus disassembly in mitosis and apoptosis: dynamic redistribution of phosphorylated-c-Myc, fibrillarin and Ki-67. EUROPEAN JOURNAL OF HISTOCHEMISTRY, vol. 50, 257-264.
19. PISU M.B, RODA E., GUIOLI S., AVELLA D., BOTTONE M.G., BERNOCCHI G. (2005) Proliferation and migration of granule cells in the developing rat cerebellum: Cisplatin effects. ANATOMICAL RECORD. PART A, DISCOVERIES IN MOLECULAR, CELLULAR, AND EVOLUTIONARY BIOLOGY. vol. 287, 1226-1235.
Project Title:
Neurotoxicity evaluation "in vitro" and "in vivo" of new platinum compounds