Daniela Carbonera
Daniela Carbonera
affiliation: Università di Pavia
research area(s): Molecular Biology, Cell Biology
Course: Genetics, Molecular and Cellular Biology
University/Istitution: Università di Pavia
Academic activity
1977 Degree in Biological Sciences at the University of Pavia
1978-1982 Awarded by a CNR fellowship at the University of Pavia
1984 Researcher at the University of Pavia
1992-1995 Teaching contract in Plant Physiology at the University of Sassari
1998-present Associate professor at the University of Pavia
Current teaching activities: Plant Physiology; Plant Biotechnology; Plant Molecular Biology and Biotechnology.
Oxidative stress and nucleus: DNA repair mechanisms in the model legume Medicago truncatula
Plant DNA is continuously exposed to damaging agents which induce a variety of DNA repair processes. Oxidative DNA damage requires prompt repair to maintain genome integrity and preserve the fidelity of genetic information. The response of plant cells to genotoxic stress relies on the activity of multiple DNA repair pathways which share some common elements with animal cells, but also own distinctive features, unique to the plant kingdom. Differently from animal systems where genotoxic agents are mainly investigated as a major cause of human cancer, genotoxic stress in plants is essentially considered as a critical factor which impairs fitness and productivity by affecting genome stability.
Oxidative DNA damage is typically associated with the accumulation of 7,8-dihydro-8-oxoguanine (8-oxo-dG), an oxidized form of guanine, which is highly mutagenic, since it frequently mispairs with the incoming dAMP during DNA replication, causing G:C to T:A transversions. The main focus of this investigation is the analysis about the contribution of Tdp1 (Tyrosyl-DNA phosphodiesterase), Tdp2, TFIIS (Transcription Elongation Factor SII) , TFIIS-like, OGG1 (8-oxoguanine DNA glycosylase/lyase), FPG (formamidopyrimidine-DNA glycosylase) genes to nuclear DNA repair under oxidative stress conditions in plants.
Molecular mechanisms induced by ionizing radiations (IR) in plant cells: application to IR-based mutation breeding in ornamentals
Genetic variability for breeding purposes is highly desired in ornamentals and IR-based mutation breeding, currently envised as a promising tool, requires optimized protocols. For this reason, novel information concerning the molecular response to IR might be used to increase the efficiency of mutation and screening for desirable phenotypes. The genus Petunia, hereby investigated, includes not only élite varieties of relevant commercial value, but also model genotypes for basic and applied research purposes. Protocols for in vitro mutagenesis of plant explants have been set up and optimized, using a 60-Cobalt source (gamma rays) with high and low dose conditions. Treatments are carried out on leaf explants from commercially relevant genotypes of Petunia x hybrida, in order to obtain flowers with novel colours and morphology. The nuclear antioxidant response, as a function of dose rate, is investigated, by analysing the levels of oxidative DNA damage, the expression profiles of DNA repair genes and free radical scavenging events. The qualitative and quantitative analysis of DNA damage are evaluated by "Single Cell Gel Electrophoresis" (Comet Assay). This research activity is carried out in collaboration with the CRA-Research Unit for Floricolture and Ornamental Species (CRA-FSO)- Sanremo and the Radiation Chemistry and EPR-Spectrometry Laboratory (Department of General Chemistry-University of Pavia).
She is co-author of 70 papers on international journals with Peer-Review.

Balestrazzi A., Confalonieri M., Macovei A., Dona’ M., Carbonera D. (2012) Genotoxic stress, DNA repair and crop productivity. In: Crop improvement under adverse conditions (N. Tuteja and SS Gill Eds), Springer-Verlag Berlin Heidelberg, in press.

Macovei A., Balestrazzi A., Confalonieri M., Faé M., Carbonera D. (2011) New insights on the barrel medic MtOGG1 and MtFPG functions in relation to oxidative stress response in planta and during seed imbibition. Plant Physiology and Biochemistry, 49: 1040-1050.

Macovei A., Balestrazzi A., Confalonieri M., Buttafava A., Carbonera D. (2011) The TFIIS-like gene from barrel medic (Medicago truncatula Gaertn.) is involved in the plant antioxidant response. Gene, 470: 20-30.

Balestrazzi A., Confalonieri M., Macovei A. , Carbonera D. (2011) Seed imbibition in Medicago truncatula Gaertn: expression profiles of DNA repair genes in relation to PEG-mediated stress. J. Plant Physiol., 168: 706-713.

Balestrazzi A., Confalonieri M., Macovei A. , Dona’ M., Carbonera D. (2011) Genotoxic stress and DNA repair in plants: emerging functions and tools for improving crop productivity. Review, Plant Cell Reports, 30: 287-295.

Balestrazzi A, Macovei A., Tava A, Avato P, Raimondi E, Carbonera D. (2011) Unraveling the response of plant cells to cytotoxic saponins: role of metallothionein and nitric oxide. Plant Signaling and Behavior, 6: 516-519.

Balestrazzi A, Bonadei M, Zelasco S, Giorcelli A, Gennaro M, Calligari P, Mattivi F, Quattrini E, Carbonera D. (2011) Seasonal and tissue-specific transgene expression and resveratrol-3-glucoside (piceid) accumulation in genetically modified white poplars carrying the grapevine StSy gene. Plant Cell, Tissue and Organ Culture, 105: 1-8.

Balestrazzi A., Agoni V., A. Tava, P. Avato, E. Biazzi, E. Raimondi, A. Macovei, D. Carbonera (2010) Cell death induction and nitric oxide biosynthesis in white poplar (Populus alba L.) suspension cultures exposed to alfalfa saponins. Pysiologia Plantarum, 141: 227-238.

Confalonieri M., Borghetti R., Macovei A., Testoni C., Carbonera D., Salema Fevereiro P., Rommens C., Swords K., Piano E., Balestrazzi A. (2010) Backbone-free transformation of barrel medic (Medicago truncatula) with a Medicago-derived transfer DNA. Plant Cell Reports, 29: 1013-1021.

Macovei A., Balestrazzi A., Confalonieri M., Carbonera D. (2010) The Tdp1 (Tyrosyl-DNA phosphodiesterase) gene family in barrel medic (Medicago truncatula Gaertn.): bioinformatic investigation and expression profiles in response to oxidative stress. Planta, 232: 393-407.

Balestrazzi A., Locato V., Bottone M.G., De Gara L., Biggioggera M., Pellicciari C., Botti S., Di Gesù D., Donà M., Carbonera D. (2010) Response to UV-C radiation in topo I-deficient carrot cells with low ascorbate levels. J. Exp. Bot., 61: 575-585.
No projects are available to students for the current accademic year.