Anna Maria Puglia
Anna Maria Puglia
affiliation: Dip. STEMBIO
research area(s): Molecular Biology, Genetics And Genomics
Course: Cell Biology
University/Istitution: Università di Palermo
Full Professor of Microbiology.
Member of Italian Society of Genetics and member of Italian Society of Microbiology and Biotechnologies (SIMGBM).
Post-doc : Genetics Lab, Istituto Superiore di Sanità, Rome; 1975
Dept. of Genetics , J.Innes Center, Norwich, England; 1988
Visiting scientist :
1992-1993: Unité de Genie Microbiologique, Pasteur Institute, Paris.
2000: Biozentrum, Basel
Research: Differentiation and stress in Streptomyces, Construction of Bacterial Artificial Chromosome Libraries, Genetics of antibiotic biosynthesis. Characterisation of soil bacteria
Since 1985 staff member of PhD course in Cell Biology and Development.
Molecular Microbiology
Microbial Biotechnology
LO PICCOLO L, DE PASQUALE C, FODALE R, PUGLIA AM, QUATRIN I(2011) P. Involvement of an alkane hydroxylase system of Gordonia sp. strain SoCg in degradation of solid n-alkanes. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 77, 1204-13.

CARDINALE M, BRUSETTI L, LANZA A, ORLANDO S, DAFFONCHIO D, PUGLIA A.M., QUATRINI P (2010). Rehabilitation of Mediterranean anthropogenic soils using symbiotic wild legume shrubs: Plant establishment and impact on the soil bacterial community structure. APPLIED SOIL ECOLOGY, ISSN: 0929-1393, doi: 10.1016/j.apsoil.2010.05.007

GALLO G, RENZONE G, ALDUINA R, STEGMANN E, WEBER T, LANTZ AE, THYKAER J, SANGIORGI F, SCALONI A, PUGLIA A.M. (2010). Differential proteomic analysis reveals novel links between primary metabolism and antibiotic production in Amycolatopsis balhimycina. PROTEOMICS, vol. 2010-01, ISSN: 1615-9853, doi: 10.1002/pmic.200900175

GALLO G, ALDUINA R, RENZONE G, THYKAER J, BIANCO L, ELIASSON-LANTZ A, SCALONI A, PUGLIA A.M. (2010). Differential proteomic analysis highlights metabolic strategies associated with balhimycin production in Amycolatopsis balhimycina chemostat cultivations. MICROBIAL CELL FACTORIES, vol. 95, ISSN: 1475-2859, doi: doi:10.1186/1475-2859-9-95

GIARDINA A, ALDUINA R, GOTTARDI E, DI CARO V, SUSSMUTH RD, PUGLIA A.M. (2010). Two heterologously expressed Planobispora rosea proteins cooperatively induce Streptomyces lividans thiostrepton uptake and storage from the extracellular medium. MICROBIAL CELL FACTORIES, vol. 9; p. 44.

TAGLIAVIA M, TARAVELLA A, MARINEO S, PUGLIA A.M., LA FARINA M (2010). Optimized RNA Extraction and Northern Hybridization in Streptomycetes. BIOLOGICAL PROCEDURES ONLINE, vol. 2010, ISSN: 1480-9222, doi: 10.1007/s12575-010-9027-7

CARDINALE M, LANZA A, BONN" M. L, MARSALA S, PUGLIA A.M., QUATRINI P (2008). Diversity of rhizobia nodulating wild shrubs of Sicily and some neighbouring Islands. ARCHIVES OF MICROBIOLOGY, vol. 190; p. 461-470.

QUATRINI P, SCAGLIONE G, DE PASQUALE C, RIELA S, PUGLIA A.M. (2008). Isolation of Gram-positive n-alkane degraders from a hydrocarbon-contaminated Mediterranean shoreline. JOURNAL OF APPLIED MICROBIOLOGY, vol. 104; p. 251-259.

ALDUINA R, LO PICCOLO L, D'ALIA D, FERRARO C, GUNNARSSON N, DONADIO S, PUGLIA A.M. (2007). A phosphate-controlled regulator for the biosynthesis of the dalbavancin precursor A40926. JOURNAL OF BACTERIOLOGY, vol. 189; p. 8120-8129.

MARINEO S, CUSIMANO MG, LIMAURO D, COTICCHIO G, PUGLIA A.M. (2007). The Histidinol Phosphate Phosphatase Involved in Histidine Biosynthetic Pathway Is Encoded by SCO5208 (hisN) in Streptomyces coelicolor A3(2). CURRENT MICROBIOLOGY, vol. 189; p. 8120-8129.

SALERNO P, MARINEO S, PUGLIA A.M. (2007). The Streptomyces coelicolor dnaK operon contains a second promoter driving the expression of the negative regulator hspR at physiological temperature. ARCHIVES OF MICROBIOLOGY, vol. 188(5); p. 541-546.

SIRAGUSA M, CARRA A, SALVIA L, PUGLIA A.M., DE PASQUALE F, CARIMI F (2007). Genetic instability in calamondin (Citrus madurensis Lour.) plants derived from somatic embryogenesis induced by diphenylurea derivatives. PLANT CELL REPORTS 26(8):1289-96.

CARDINALE M, PUGLIA A.M., GRUBE M (2006). Molecular analysis of lichen-associated bacterial communities. FEMS MICROBIOLOGY ECOLOGY, vol. 57; p. 484-495.

ALDUINA R., GIARDINA A., GALLO G., RENZONE G., FERRARO C., CONTINO A., SCALONI A., DONADIO S., PUGLIA A.M. (2005). Expression in Streptomyces lividans of Nonomuraea genes cloned in an artificial chromosome. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 68; p. 656-662.

MARINEO S., LECAT E., CUSIMANO M.G., GIARDINA A., DI CARO V., PUGLIA A.M. (2005). Identification of SCP2165, a new SCP2-derived plasmid of Streptomyces coelicolor A3(2). LETTERS IN APPLIED MICROBIOLOGY, vol. 41; p. 350-354.

CARDINALE M., BRUSETTI L., QUATRINI P., BORIN S., PUGLIA A.M., RIZZI A., ZANARDINI E., SORLINI C., CORSELLI C., DAFFONCHIO D. (2004). Comparison of different primer sets for use in automated ribosomal intergenic spacer analysis of complex bacterial communities. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 70; p. 6147-6156.

ALDUINA R., DE GRAZIA S., DOLCE L., SALERNO P., SOSIO M., DONADIO S., PUGLIA A.M. (2003). Artificial Chromosome Libraries of Streptomyces coelicolor and Planobispora rosea. FEMS MICROBIOLOGY LETTERS, vol. 218; p. 179-184.

QUATRINI P., GENTILE M., CARIMI F., DE PASQUALE F., PUGLIA A.M. (2003). Effect of native arbuscular mycorrhizal fungi and Glomus mosseae on acclimatization and development of micropropagated Citrus Limon (L.) Burm. JOURNAL OF HORTICULTURAL SCIENCE AND BIOTECHNOLOGY, vol. 78; p. 39-45.

QUATRINI P., SCAGLIONE G., INCANNELLA G., PUGLIA A.M., LA MANTIA T. (2003). Microbial inoculants on woody legumes to recover a municipal landfill site. WATER AIR AND SOIL POLLUTION; p. 189-199.

Project Title:
Characterization of Streptomyces small ORFs
Streptomycetes are non-motile, filamentous, Gram positive bacteria. Streptomycetes are remarkable in terms of the morphological and metabolic differentiation phenomena that they present. Streptomyces spp. generally synthesise a large number of very diverse secondary metabolites, the best known being antibiotics but others have antifungal, anti-parasitic, anti-tumoural, immunosuppressant, antiviral, insecticides or herbicides activity.
In Streptomyces several short RNAs encode small proteins. Although small proteins have largely been missed, the few small proteins that have been studied in detail in bacterial and mammalian cells have been shown to have important functions in both signaling and cellular defenses. Thus, a project was established to elucidate the role of Streptomyces coelicolor small proteins, using many of the approaches used to characterize the functions of several actinomycete proteins involved in aminoacid and antibiotic biosynthesis regulation.