Fabio Caradonna
e-mail: fabio.caradonna AT unipa.it
website: www.unipa.it/fabio.caradonna/
affiliation: Dip. STEMBIO
research area(s): Genetics And Genomics, Cell Biology
Course:
Cell Biology
University/Istitution: Università di Palermo
University/Istitution: Università di Palermo
Biological Sciences University Degree in Biological Sciences 110/110 with maximum honours
Qualification to work as biologist.
PhD in Cellular and Developmental Biology
National qualifying examination and teaching diploma for secondary schools
Specialist in Clinical Pathology
English language diploma (second level and admission to the third level:pre-intermediate-intermediate)
Diploma in "Bioethics and culture of life" at the Papal University of Santa Croce, Rome.
Patent cultures for the use of cloned human DNA in procariotic organisms.
Qualification to work as biologist.
PhD in Cellular and Developmental Biology
National qualifying examination and teaching diploma for secondary schools
Specialist in Clinical Pathology
English language diploma (second level and admission to the third level:pre-intermediate-intermediate)
Diploma in "Bioethics and culture of life" at the Papal University of Santa Croce, Rome.
Patent cultures for the use of cloned human DNA in procariotic organisms.
Excellent experience in:
Cytogenetics, molecular cytogenetics, genotyping of genic polymorphisms, DNA methylation status technics, RT-PCR with taqman chemistry
Supervisor for about 50 experimental thesis on Genetics
Permanent researcher of Genetics, University of Palermo
Permanent teacher of Evolutionary Genetics, Genetics of populations, Molecular Genetics, Plant Genetics at Biological Sciences course of studies, University of Palermo.Member of both the scientific and organizing committee of a lot of refresher course, some of these granted by Health Ministry with credits within the Long-life Learning in Medicine (ECM).
Cytogenetics, molecular cytogenetics, genotyping of genic polymorphisms, DNA methylation status technics, RT-PCR with taqman chemistry
Supervisor for about 50 experimental thesis on Genetics
Permanent researcher of Genetics, University of Palermo
Permanent teacher of Evolutionary Genetics, Genetics of populations, Molecular Genetics, Plant Genetics at Biological Sciences course of studies, University of Palermo.Member of both the scientific and organizing committee of a lot of refresher course, some of these granted by Health Ministry with credits within the Long-life Learning in Medicine (ECM).
Coauthor of 14 ISI papers, 2 Italian national papers ISSN, 2 book chapter ISBN, 1 book ISBN, 8 international abstracts ISI published and 38 national and international abstracts not ISI published. The most important:
1- "Early induction of genetic instability and apoptosis by arsenic in cultured Chinese hamster cells". Sciandrello G, Barbaro R, Caradonna F., Barbata G. Mutagenesis, 17 (2), 99-103 2002 (Impact factor: 1,538).
2- "The DNA methylation inhibitor 5-azacytidine modulates 6-thioguanine toxicity in mammalian cells". Sciandrello G. Caradonna F. Barbata G. Toxicology Letters, 142, 1-2, 30, 29 " 35, 2003 (Impact factor: 2,242).
3- "Arsenic-induced DNA hypomethylation affects chromosomal instability in mammalian cells". Sciandrello G, Caradonna F, Barbata G. Carcinogenesis (Impact factor:4,543) 25, 413-417, 2004.
4- "Mid-region parathyroid hormone-related protein (PTHrP) binds chromatin of MDA-MB231 breast cancer cells and isolated oligonucleotides "in vitro"". Sirchia R, Priulla M, Sciandrello G, Caradonna F., Barbata G, Luparello C. Breast Cancer Research and Treatment (Impact Factor: 4,64), Nov., 24, 2006.
5- "Chromosomal localization and molecular characterization of three different 5S ribosomal DNA clusters in the sea urchin Paracentrotus Lividus". Fabio Caradonna, Daniele Bellavia, Ann Maria Clemente, Giorgia Sisino and Rainer Barbieri. Genome (Impact factor: 1,86) vol. 50, 867-870, 2007.
6- "Acrylamide catalytically inhibits topoisomerase II in V79 cells". Sciandrello G, Mauro M, Caradonna F., Catanzaro I, Saverini M, Barbata G. Toxicology in vitro (Impact factor: 2,473) vol. 00, ISSN: 0887-2333, doi: 10.1016, 2009.
7- "Biological effects of inorganic arsenic on primary cultures of rat astrocytes". Irene Catanzaro, Gabriella Schiera, Giulia Sciandrello, Giusi Barbata, Fabio Caradonna, Patrizia Proia and Italia Di Liegro. International Journal of Molecular Medicine, vol. 26; p. 457-462, ISSN: 1107-3756, doi: 10.3892/ijmm_00000485, 2010.
8-"Long-Lasting Genomic Instability Following Arsenite Exposure inMammalian Cells: The Role of Reactive Oxygen Species". Giulia Sciandrello, Maurizio Mauro, Irene Catanzaro, Marghereth Saverini, Fabio Caradonna & Giusi Barbata. Environmental and molecular mutagenesis, Apr 25. doi: 10.1002/em.20657[Epub ahead of print], 2011.
1- "Early induction of genetic instability and apoptosis by arsenic in cultured Chinese hamster cells". Sciandrello G, Barbaro R, Caradonna F., Barbata G. Mutagenesis, 17 (2), 99-103 2002 (Impact factor: 1,538).
2- "The DNA methylation inhibitor 5-azacytidine modulates 6-thioguanine toxicity in mammalian cells". Sciandrello G. Caradonna F. Barbata G. Toxicology Letters, 142, 1-2, 30, 29 " 35, 2003 (Impact factor: 2,242).
3- "Arsenic-induced DNA hypomethylation affects chromosomal instability in mammalian cells". Sciandrello G, Caradonna F, Barbata G. Carcinogenesis (Impact factor:4,543) 25, 413-417, 2004.
4- "Mid-region parathyroid hormone-related protein (PTHrP) binds chromatin of MDA-MB231 breast cancer cells and isolated oligonucleotides "in vitro"". Sirchia R, Priulla M, Sciandrello G, Caradonna F., Barbata G, Luparello C. Breast Cancer Research and Treatment (Impact Factor: 4,64), Nov., 24, 2006.
5- "Chromosomal localization and molecular characterization of three different 5S ribosomal DNA clusters in the sea urchin Paracentrotus Lividus". Fabio Caradonna, Daniele Bellavia, Ann Maria Clemente, Giorgia Sisino and Rainer Barbieri. Genome (Impact factor: 1,86) vol. 50, 867-870, 2007.
6- "Acrylamide catalytically inhibits topoisomerase II in V79 cells". Sciandrello G, Mauro M, Caradonna F., Catanzaro I, Saverini M, Barbata G. Toxicology in vitro (Impact factor: 2,473) vol. 00, ISSN: 0887-2333, doi: 10.1016, 2009.
7- "Biological effects of inorganic arsenic on primary cultures of rat astrocytes". Irene Catanzaro, Gabriella Schiera, Giulia Sciandrello, Giusi Barbata, Fabio Caradonna, Patrizia Proia and Italia Di Liegro. International Journal of Molecular Medicine, vol. 26; p. 457-462, ISSN: 1107-3756, doi: 10.3892/ijmm_00000485, 2010.
8-"Long-Lasting Genomic Instability Following Arsenite Exposure inMammalian Cells: The Role of Reactive Oxygen Species". Giulia Sciandrello, Maurizio Mauro, Irene Catanzaro, Marghereth Saverini, Fabio Caradonna & Giusi Barbata. Environmental and molecular mutagenesis, Apr 25. doi: 10.1002/em.20657[Epub ahead of print], 2011.
Project Title:
Nutrigenomic effects of Indicaxantin in cell lines: mechanism of action and its influence on gene expression
Nutrigenomics is an emerging science which aims to identify and study the diet molecules having influence on beginning of some diseases and to characterize their possible mechanisms of action. In particular it intends to understand the nutrient-genome interaction, how diet molecules are able to interfere with DNA.
Phytochemicals are biologically active molecules in vegetables and are an highly inhomogeneous cluster of substances with protective effects on human health. Phytochemical Indicaxantin, in particular, is a yellow pigment contained in the mediterranean plant Opunzia ficus indica fruit.
It was recently demonstrated that indicaxantin have antioxidant properties, E vitamin-like and it was also demonstrated that pathogenesis of alcohol-consumption diseases is similar to an inflammatory process (Weise JG et al. 2000). Some phytochemical are able to modulate gene expression of metabolizing enzymes, like CYP2E1 for example, and, Opunzia ficus indica fruit-extract reduces symptoms of acute alcohol consumption (Wiese JG et al. 2004).
Nothing, unfortunately, was reported about cause/effect relation between indicaxantin and expression modulation of xeno-methabilizing genes, so, it could be more interesting to clarify the gene-regulating role of indicaxantin, by the understanding of its mechanism of action.
In conclusion the present project aim to evaluate nutrigenomic properties of indicaxantin in hepatic and non-hepatic cell lines to understand its protective mechanisms, taking also into consideration a role of direct or indirect gene expression regulator.
Results from this project could give the basis for probable future use of indicaxantin in phytotherapy.
Phytochemicals are biologically active molecules in vegetables and are an highly inhomogeneous cluster of substances with protective effects on human health. Phytochemical Indicaxantin, in particular, is a yellow pigment contained in the mediterranean plant Opunzia ficus indica fruit.
It was recently demonstrated that indicaxantin have antioxidant properties, E vitamin-like and it was also demonstrated that pathogenesis of alcohol-consumption diseases is similar to an inflammatory process (Weise JG et al. 2000). Some phytochemical are able to modulate gene expression of metabolizing enzymes, like CYP2E1 for example, and, Opunzia ficus indica fruit-extract reduces symptoms of acute alcohol consumption (Wiese JG et al. 2004).
Nothing, unfortunately, was reported about cause/effect relation between indicaxantin and expression modulation of xeno-methabilizing genes, so, it could be more interesting to clarify the gene-regulating role of indicaxantin, by the understanding of its mechanism of action.
In conclusion the present project aim to evaluate nutrigenomic properties of indicaxantin in hepatic and non-hepatic cell lines to understand its protective mechanisms, taking also into consideration a role of direct or indirect gene expression regulator.
Results from this project could give the basis for probable future use of indicaxantin in phytotherapy.