Rosa Alduina
e-mail: valduina AT unipa.it
affiliation: Dip. STEMBIO
research area(s): Molecular Biology, Genetics And Genomics
Course:
Cell Biology
University/Istitution: Università di Palermo
University/Istitution: Università di Palermo
2004 PhD in Cellular and Developmental Biology, D.B.C.S, University of Palermo.
2006 Researcher in Microbiology, University of Palermo.
2008 Visiting scientist, Dipartimento di Biologia Molecolare Cellulare e Animale of Camerino University, (Prof. C. Gualerzi) 20th november-12nd december.
2009 Visiting scientist, Department of Molecular Microbiology, John Innes Centre, Norwich, UK (Prof. M. Bibb) 14th january-15th may.
2006 Researcher in Microbiology, University of Palermo.
2008 Visiting scientist, Dipartimento di Biologia Molecolare Cellulare e Animale of Camerino University, (Prof. C. Gualerzi) 20th november-12nd december.
2009 Visiting scientist, Department of Molecular Microbiology, John Innes Centre, Norwich, UK (Prof. M. Bibb) 14th january-15th may.
Molecular Microbiology
Microbial Biotechnology
Microbial Biotechnology
- Gallo G, Alduina R, Renzone G, Thykaer J, Bianco L, Eliasson-Lantz A, Scaloni A, Puglia AM. (2010) Differential proteomic analysis highlights metabolic strategies associated with balhimycin production in Amycolatopsis balhimycina chemostat cultivations. Microb Cell Fact.; 9:95.
- Siculella L, Damiano F, di Summa R, Tredici SM, Alduina R, Gnoni GV, Alifano P. (2010) Guanosine 5'-diphosphate 3'-diphosphate (ppGpp) as a negative modulator of polynucleotide phosphorylase activity in a 'rare' actinomycete. Mol Microbiol. 77(3):716-29.
- Giardina A, Alduina R, Gottardi E, Di Caro V, Süssmuth RD, Puglia AM. (2010) Two heterologously expressed Planobispora rosea proteins cooperatively induce Streptomyces lividans thiostrepton uptake and storage from the extracellular medium. Microb Cell Fact.; 9:44.
- Gallo G, Renzone G, Alduina R, Stegmann E, Weber T, Lantz AE, Thykaer J, Sangiorgi F, Scaloni A, Puglia AM. (2010) Differential proteomic analysis reveals novel links between primary metabolism and antibiotic production in Amycolatopsis balhimycina. Proteomics.; 10(7):1336-58.
- Alduina R, Lo Piccolo L, D'Alia D, Ferraro C, Gunnarsson N, Donadio S, Puglia AM. (2007) Phosphate-controlled regulator for the biosynthesis of the dalbavancin precursor A40926. J Bacteriol.;189(22):8120-9.
- Alduina R, Giardina A, Gallo G, Renzone G, Ferraro C, Contino A, Scaloni A, Donadio S, Puglia AM. (2005) Expression in Streptomyces lividans of Nonomuraea genes cloned in an artificial chromosome. Appl Microbiol Biotechnol. 68(5):656-62.
- Alduina R, De Grazia S, Dolce L, Salerno P, Sosio M, Donadio S, Puglia AM. (2003) Artificial chromosome libraries of Streptomyces coelicolor A3(2) and Planobispora rosea. FEMS Microbiol Lett.; 218(1):181-6.
- Donadio S, Monciardini P, Alduina R, Mazza P, Chiocchini C, Cavaletti L, Sosio M, Puglia AM. (2002) Microbial technologies for the discovery of novel bioactive metabolites. J Biotechnol.; 99(3):187-98.
- Siculella L, Damiano F, di Summa R, Tredici SM, Alduina R, Gnoni GV, Alifano P. (2010) Guanosine 5'-diphosphate 3'-diphosphate (ppGpp) as a negative modulator of polynucleotide phosphorylase activity in a 'rare' actinomycete. Mol Microbiol. 77(3):716-29.
- Giardina A, Alduina R, Gottardi E, Di Caro V, Süssmuth RD, Puglia AM. (2010) Two heterologously expressed Planobispora rosea proteins cooperatively induce Streptomyces lividans thiostrepton uptake and storage from the extracellular medium. Microb Cell Fact.; 9:44.
- Gallo G, Renzone G, Alduina R, Stegmann E, Weber T, Lantz AE, Thykaer J, Sangiorgi F, Scaloni A, Puglia AM. (2010) Differential proteomic analysis reveals novel links between primary metabolism and antibiotic production in Amycolatopsis balhimycina. Proteomics.; 10(7):1336-58.
- Alduina R, Lo Piccolo L, D'Alia D, Ferraro C, Gunnarsson N, Donadio S, Puglia AM. (2007) Phosphate-controlled regulator for the biosynthesis of the dalbavancin precursor A40926. J Bacteriol.;189(22):8120-9.
- Alduina R, Giardina A, Gallo G, Renzone G, Ferraro C, Contino A, Scaloni A, Donadio S, Puglia AM. (2005) Expression in Streptomyces lividans of Nonomuraea genes cloned in an artificial chromosome. Appl Microbiol Biotechnol. 68(5):656-62.
- Alduina R, De Grazia S, Dolce L, Salerno P, Sosio M, Donadio S, Puglia AM. (2003) Artificial chromosome libraries of Streptomyces coelicolor A3(2) and Planobispora rosea. FEMS Microbiol Lett.; 218(1):181-6.
- Donadio S, Monciardini P, Alduina R, Mazza P, Chiocchini C, Cavaletti L, Sosio M, Puglia AM. (2002) Microbial technologies for the discovery of novel bioactive metabolites. J Biotechnol.; 99(3):187-98.
Project Title:
Regulation of antibiotic biosynthesis in the actinomycete Nonomuraea sp. ATCC 39727
Actinomycetes produce two-thirds of the clinically useful antibiotics of natural origin. Therefore, considerable efforts are directed towards understanding how these bacteria synthesize antibiotics, in order to enhance antibiotic production trough the rational engineering of strains.
Biosynthesis of antibiotics is a complex process involving several levels of regulation in a highly tight manner.
The actinomycete Nonomuraea produces the glycopeptide A40926, precursor of dalbavancin. Dalbavancin is a novel lipoglycopeptide agent against emerging resistant Gram-positive cocci with superior pharmacodynamics properties compared to vancomycin .
The A40926 biosynthetic gene (dbv) cluster encodes the regulators Dbv3 (LuxR-like) and Dbv4 (StrR-like), as well as the putative response regulator Dbv6 and the sensor-kinase Dbv22, that may be part of a two-component system.
Up to now, only the role of Dbv4, a StrR-like protein, was elucidated. Indeed, Dbv4 positively regulates the transcription of two operons, dbv14-8 and dbv30-35 encoding structural proteins (Alduina et al. J Bacteriol. 2007).
The aim of the project is to determine the role of Dbv3 and Dbv6 in A40926 biosynthesis by genetic and biochemical approaches.
These studies will elucidate the complex regulatory network governing antibiotic production in actinomycetes and will offer a platform for the manipulation of Nonomuraea for increased production of A40926.
Biosynthesis of antibiotics is a complex process involving several levels of regulation in a highly tight manner.
The actinomycete Nonomuraea produces the glycopeptide A40926, precursor of dalbavancin. Dalbavancin is a novel lipoglycopeptide agent against emerging resistant Gram-positive cocci with superior pharmacodynamics properties compared to vancomycin .
The A40926 biosynthetic gene (dbv) cluster encodes the regulators Dbv3 (LuxR-like) and Dbv4 (StrR-like), as well as the putative response regulator Dbv6 and the sensor-kinase Dbv22, that may be part of a two-component system.
Up to now, only the role of Dbv4, a StrR-like protein, was elucidated. Indeed, Dbv4 positively regulates the transcription of two operons, dbv14-8 and dbv30-35 encoding structural proteins (Alduina et al. J Bacteriol. 2007).
The aim of the project is to determine the role of Dbv3 and Dbv6 in A40926 biosynthesis by genetic and biochemical approaches.
These studies will elucidate the complex regulatory network governing antibiotic production in actinomycetes and will offer a platform for the manipulation of Nonomuraea for increased production of A40926.