Patrizia Rovere-Querini
Patrizia Rovere-Querini
affiliation: San Raffaele Scientific Institute
research area(s): Stem Cells And Regenerative Medicine, Immunity And Infection
Course: Basic and Applied Immunology
University/Istitution: Università Vita-Salute San Raffaele

University of Milano, Italy M.D. 1994
Open University, London Ph.D. 2000
Vita-Salute University Milano, Italy Degree in Allergy and Clinical Immunology 2002

Positions and Employment
1994-1995 Fellow, Laboratory of Tumor Immunology, Scientific Institute H S. Raffaele, Milano, Italy
1995-1997 Postdoctoral Fellow, Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, Marseille, France.
1998-2008 Scientist, Cancer Immunotherapy and Gene Therapy Program, DIBIT, Scientific Institute H S. Raffaele, Milano, Italy.
2008-date Group Leader, Division of Regenerative Medicine, DIBIT, Scientific Institute H S. Raffaele, Milano, Italy.

1988 & 1995 A. Origlia Award, Ente del Commercio, Milano
1998 Roche Award for the Research in Immunology, Italian Society of Immunology.
1999 Cecilia Cioffrese Award, Carlo Erba Foundation.
2000 Internal Medicine Award, Aventis Foundation and Italian Society of Internal Medicine.

Main Research Interests:

Immune pathways regulating the remodeling and regeneration of injured tissues, with specific attention to the skeletal muscle and the peritoneal lining.

Triggers of sterile inflammation in persistent human diseases
Selected recent publications (2007-2011)

1. Vezzoli M, Castellani P, Corna G, Castiglioni A, Bosurgi L, Monno A, Brunelli S, Manfredi AA, Rubartelli A, Rovere-Querini P. High-Mobility Group Box 1 Release and Redox Regulation Accompany Regeneration and Remodeling of Skeletal Muscle. Antioxid Redox Signal. 2011 in press
2. Manfredi AA, Rovere-Querini P. The mitochondrion--a Trojan horse that kicks off inflammation? N Engl J Med. 2010 Jun 3;362(22):2132-4.
3. Manfredi AA, Rovere-Querini P, Maugeri N. Dangerous connections: neutrophils and the phagocytic clearance of activated platelets. Curr Opin Hematol. 2010 Jan;17(1):3-8. Review.
4. Vezzoli M, Castellani P, Campana L, Corna G, Bosurgi L, Manfredi AA, Bianchi ME, Rubartelli A, Rovere-Querini P.Redox remodeling: a candidate regulator of HMGB1 function in injured skeletal muscle. Ann N Y Acad Sci. 2010 Oct;1209:83-90.
5. Corna G, Campana L, Pignatti E, Castiglioni A, Tagliafico E, Bosurgi L, Campanella A, Brunelli S, Manfredi AA, Apostoli P, Silvestri L, Camaschella C, Rovere-Querini P. Polarization dictates iron handling by inflammatory and alternatively activated macrophages. Haematologica. 2010 Nov;95(11):1814-22.
6. Bacci M, Capobianco A, Monno A, Cottone L, Di Puppo F, Camisa B, Mariani M, Brignole C, Ponzoni M, Ferrari S, Panina-Bordignon P, Manfredi AA, Rovere-Querini P. Macrophages are alternatively activated in patients with endometriosis and required for growth and vascularization of lesions in a mouse model of disease. Am J Pathol. 2009 Aug;175(2):547-56.
7. Campana L, Bosurgi L, Bianchi ME, Manfredi AA, Rovere-Querini P. Requirement of HMGB1 for stromal cell-derived factor-1/CXCL12-dependent migration of macrophages and dendritic cells. J Leukoc Biol. 2009; Sep;86(3):609-15.
8. Manfredi AA, Capobianco A, Bianchi ME, Rovere-Querini P. Regulation of dendritic- and T-cell fate by injury-associated endogenous signals. Crit Rev Immunol. 2009; 29:69-86.
9. Maugeri N, Rovere-Querini P, Baldini M, Sabbadini MG, Manfredi AA. Translational mini-review series on immunology of vascular disease: mechanisms of vascular inflammation and remodelling in systemic vasculitis. Clin Exp Immunol. 2009 Jun;156(3):395-404.
10. Maugeri N, Rovere-Querini P, Evangelista V, Covino C, Capobianco A, Bertilaccio MT, Piccoli A, Totani L, Cianflone D, Maseri A, Manfredi AA. Neutrophils phagocytose activated platelets in vivo: a phosphatidylserine, P-selectin, and {beta}2 integrin-dependent cell clearance program. Blood. 2009 May 21;113(21):5254-65.
11. Lolmede K, Campana L, Vezzoli M, Bosurgi L, Tonlorenzi R, Clementi E, Bianchi ME, Cossu G, Manfredi AA, Brunelli S, Rovere-Querini P. Inflammatory and alternatively activated human macrophages attract vessel-associated stem cells, relying on separate HMGB1- and MMP-9-dependent pathways. J Leukoc Biol. 2009 May;85(5):779-87.
12. Urbonaviciute V, Fürnrohr BG, Meister S, Munoz L, Heyder P, De Marchis F, Bianchi ME, Kirschning C, Wagner H, Manfredi AA, Kalden JR, Schett G, Rovere-Querini P, Herrmann M, Voll RE. Induction of inflammatory and immune responses by HMGB1-nucleosome complexes: implications for the pathogenesis of SLE. J Exp Med. 2008 Dec 22;205(13):3007-18.
13. Lolmède K, Rovere-Querini P. [Role of HMGB1 in the induction of immune responses against dying cells]. Pathol Biol (Paris). 2009 May;57(3):217-8.
14. Brunelli S, Rovere-Querini P. The immune system and the repair of skeletal muscle. Pharmacol Res. 2008 Aug;58(2):117-21.
15. Campana L, Bosurgi L, Rovere-Querini P. HMGB1: a two-headed signal regulating tumor progression and immunity. Curr Opin Immunol. 2008 Oct;20(5):518-23.
16. Manfredi AA, Rovere-Querini P, Bottazzi B, Garlanda C, Mantovani A. Pentraxins, humoral innate immunity and tissue injury. Curr Opin Immunol. 2008 Oct;20(5):538-44.
17. Manfredi AA, Capobianco A, Esposito A, De Cobelli F, Canu T, Monno A, Raucci A, Sanvito F, Doglioni C, Nawroth PP, Bierhaus A, Bianchi ME, Rovere-Querini P, Del Maschio A. Maturing Dendritic Cells Depend on RAGE for In Vivo Homing to Lymph Nodes. J Immunol. 2008 Feb 15;180(4):2270-5.
18. Rovere-Querini P, Brunelli S, Clementi E, Manfredi AA. Cell death: tipping the balance of autoimmunity and tissue repair. Curr Pharm Des. 2008;14:269-77.
19. Di Comite G, Grazia Sabbadini M, Corti A, Rovere-Querini P, Manfredi AA. Conversation galante: How the immune and the neuroendocrine systems talk to each other. Autoimmun Rev. 2007, 7:23-9.
20. Perrotta C, Bizzozero L, Falcone S, Rovere-Querini P, Prinetti A, Schuchman EH, Sonnino S, Manfredi AA, Clementi E. Nitric oxide boosts chemoimmunotherapy via inhibition of acid sphingomyelinase in a mouse model of melanoma. Cancer Res. 2007, 67:7559-64.

Project Title:
Role of skeletal muscle regeneration impairment in Inflammatory muscle diseases

Idiopathic Inflammatory Myopathies (IIM) are characterized by inflammatory
involvement of the proximal skeletal muscle with T cell infiltrates and
autoantibody generation. Target autoantigens are involved in gene
transcription/protein synthesis and translocation such as aminoacyl-tRNA
synthetases (e.g. Jo-1) and are highly expressed into skeletal muscles. Immune-
mediated loss of muscle fibers is the main mechanisms of muscle weakness.
Adult skeletal muscle regenerates upon damage by activation of resident satellite
cells that proliferate and differentiate to form new fibers.
We will investigate if reduced muscle function and weakness in IIM involves an
impairment of satellite cell functions and if cell therapy may be feasible.
The project will be divided into three phases:
1. Analysis of Jo-1 expression in satellite cells during skeletal muscle
regeneration induced by injuries that causes different inflammatory
reaction (carditoxin or glycerol injection, forced exercise, cancer-induced
atrophy). The effect of Jo-1 on their in vitro proliferation and
differentiation capacity will be also investigated.
2. Generation of mouse model of IMM by injection of cloned Jo-1 protein to
study satellite cells during disease progression and after exercise-induced
damage. Experiments will be also performed in DO11.10/RAG-2-/- mice to
study the effect of innate immunity on satellite cells.
3. Study of therapeutic efficacy of donor satellite cells injections (or other
stem cells) in the mouse model of the disease.

Sciorati C, et al Necdin is expressed in cachectic skeletal muscle to protect
fibers from tumor-induced wasting. J Cell Sci. 2009 Apr 15;122(Pt 8):1119-25.
Vezzoli M, et al High-Mobility Group Box 1 Release and Redox Regulation
Accompany Regeneration and Remodeling of Skeletal Muscle. Antioxid Redox
Signal. 2011 in press