Claudio Santoro
Claudio Santoro
affiliation: Università del Piemonte Orientale
research area(s): Molecular Biology, Genetics And Genomics
Course: Biotechnologies For Human Health
University/Istitution: Università del Piemonte Orientale
Degree :
1981 M.D. at the University of Turin " Italy

Positions and Employment
1984 Research Assistant, Department of Biology Chemistry and Genetics, University of Turin (Italy)
1992 Associate Professor of Biology, Department of Biochemistry, University of Trieste (Italy)
1995 Associate Professor of Biology, Department of Medical Sciences, University of Eastern Piedmont "A. Avogadro", Novara (Italy)
2000- Professor of Biology, Department of Medical Sciences, University of Easter Piedmont "A. Avogadro", Novara (Italy)

Other Experience and Professional Memberships
1981-1982 Research Fellow, Department of Nephrology, Guy"s Hospital London (UK)
1982-1984 Research Fellow, European Molecular Biology Laboratory, Heidelberg (Germany)
1986-1989 Research Visitor, Department of Biochemistry, University of California Berkeley (USA)
1995 Member of the Italian Society for Biology and Genetics
1992-1996 Group Leader, National Laboratory of Biotechnology (LN-CIB), Trieste (Italy)
2002 Member of the Scientific Committee, Interdisciplinary Research Center for Autoimmune Diseases (IRCAD)
2005 Coordinator of the PhD program in "Biotechnologies for Human Health"

Honours and fellowships
1981-1982 Villa Rusconi fellowship
1982-1984 EMBL postdoctoral fellowship.
1986-1988 EMBO postdoctoral fellowship.
1988-1989 HHMI fellow

Funding Sources
CNR Strategic project "Biotechnologies"
EU " FP4 FP6
Cariplo Foundation
Compagnia San Paolo

Scientific collaborations
Prof. R.Marzari, Dip. Biologia, Università degli Studi di Trieste, Trieste, (Italy).
Prof. D. Sblattero, University of Eastern Piedmont "A. Avogadro", Novara (Italy)
Prof. J. Marks, UCSF, California, (USA).
Prof, A. M. Bradbury, LANL, New Mexico, (USA)
Prof. Costantino Pitzalis, London (UK)
Development of protein microarray technology for biomarkers dicovery.
Phage display and derived technologies.
Gene expression regulation.
Interactome profiling.
1. Cotella D, Radicke S, Cipriani V, Cavaletto M, Merlin S, Follenzi A, Ravens U, Wettwer E, Santoro C, Sblattero D.N-glycosylation of the mammalian dipeptidyl aminopeptidase-like protein 10 (DPP10) regulates trafficking and interaction with Kv4 channels. Int J Biochem Cell Biol. 2012 44(6):876-85.

2. Boscolo S, Mion F, Licciulli M, Macor P, De Maso L, Brce M, Antoniou MN, Marzari R, Santoro C, Sblattero D.Simple scale-up of recombinant antibody production using an UCOE containing vector.N Biotechnol. 2012 15;29(4):477-84.

3. Romeo F, Falbo L, Di Sanzo M, Misaggi R, Faniello MC, Barni T, Cuda G, Viglietto G, Santoro C, Quaresima B, Costanzo F. Negative transcriptional regulation of the human periostin gene by YingYang-1 transcription factor. Gene. 2011 Nov 10;487(2):129-34.

4. D'Angelo S, Velappan N, Mignone F, Santoro C, Sblattero D, Kiss C, Bradbury AR. Filtering "genic" open reading frames from genomic DNA samples for advanced annotation. BMC Genomics. 2011 Jun 15;12 Suppl 1:S5.

5. Zucchelli S, Marcuzzi F, Codrich M, Agostoni E, Vilotti S, Biagioli M, Pinto M, Carnemolla A, Santoro C, Gustincich S, Persichetti F. Tumor necrosis factor receptor-associated factor 6 (TRAF6) associates with huntingtin protein and promotes its atypical ubiquitination to enhance aggregate formation.J Biol Chem. 2011 Jul 15;286(28):25108-17.

6. Cotella D, Radicke S, Bortoluzzi A, Ravens U, Wettwer E, Santoro C, Sblattero D. Impaired glycosylation blocks DPP10 cell surface expression and alters the electrophysiology of Ito channel complex. Pflugers Arch. 2010 Jun;460(1):87-97.

7. Di Niro R, Sulic AM, Mignone F, D'Angelo S, Bordoni R, Iacono M, Marzari R, Gaiotto T, Lavric M, Bradbury AR, Biancone L, Zevin-Sonkin D, De Bellis G, Santoro C, Sblattero D. Rapid interactome profiling by massive sequencing. Nucleic Acids Res. 2010 May;38(9):e110.

8. Ferretti M, Gattorno M, Chiocchetti A, Mesturini R, Orilieri E, Bensi T, Sormani MP, Cappellano G, Cerutti E, Nicola S, Biava A, Bardelli C, Federici S, Ceccherini I, Baldi M, Santoro C, Dianzani I, Martini A, Dianzani U. The 423Q polymorphism of the X-linked inhibitor of apoptosis gene influences monocyte function and is associated with periodic fever. Arthritis Rheum. 2009 Nov;60(11):3476-84.

9. Avondo F, Roncaglia P, Crescenzio N, Krmac H, Garelli E, Armiraglio M, Castagnoli C, Campagnoli MF, Ramenghi U, Gustincich S, Santoro C, Dianzani I.Fibroblasts from patients with Diamond-Blackfan anaemia show abnormal expression of genes involved in protein synthesis, amino acid metabolism and cancer. BMC Genomics. 2009 Sep 18;10:442.

10. Biagioli M, Pinto M, Cesselli D, Zaninello M, Lazarevic D, Roncaglia P, Simone R, Vlachouli C, Plessy C, Bertin N, Beltrami A, Kobayashi K, Gallo V, Santoro C, Ferrer I, Rivella S, Beltrami CA, Carninci P, Raviola E, Gustincich S.Unexpected expression of alpha- and beta-globin in mesencephalic dopaminergic neurons and glial cells. Proc Natl Acad Sci U S A. 2009 Sep 8;106(36):15454-9.

11. Di Niro R, D'Angelo S, Secco P, Marzari R, Santoro C, Sblattero D. Profiling the autoantibody repertoire by screening phage-displayed human cDNA libraries. Methods Mol Biol. 2009;570:353-69. Review.

12. Radicke S, Cotella D, Sblattero D, Ravens U, Santoro C, Wettwer E.The transmembrane beta-subunits KCNE1, KCNE2, and DPP6 modify pharmacological effects of the antiarrhythmic agent tedisamil on the transient outward current Ito. Naunyn Schmiedebergs Arch Pharmacol. 2009 Jun;379(6):617-26.

13. Secco P, D'Agostini E, Marzari R, Licciulli M, Di Niro R, D'Angelo S, Bradbury AR, Dianzani U, Santoro C, Sblattero D.Antibody library selection by the {beta}-lactamase protein fragment complementation assay. Protein Eng Des Sel. 2009 Mar;22(3):149-58.

14. Boria I, Cotella D, Dianzani I, Santoro C, Sblattero D.Primer sets for cloning the human repertoire of T cell Receptor Variable regions. BMC Immunol. 2008 Aug 29;9:50.

15. Giordano M, Godi M, Mellone S, Petri A, Vivenza D, Tiradani L, Carlomagno Y, Ferrante D, Arrigo T, Corneli G, Bellone S, Giacopelli F, Santoro C, Bona G, Momigliano-Richiardi P. A functional common polymorphism in the vitamin D-responsive element of the GH1 promoter contributes to isolated growth hormone deficiency. J Clin Endocrinol Metab. 2008 Mar;93(3):1005-12.
Project Title:
Exploiting ORF display technology to profile RNA interactome
Genome-wide surveys have revealed that the majority of the mammalian genome is transcribed in a vast repertoire of transcripts that includes protein-encoding mRNAs, long non-coding RNA (lncRNA) and transcribed repetitive sequences. Antisense (AS) lncRNAs may participate to sense/antisense pairs (S/AS) overlapping with a protein coding gene on the opposite strand and regulate epigenetic silencing, transcription and mRNA stability. Some AS lncRNAs have been identified to increases the protein synthesis encoded by the paired S RNA at a post-transcriptional level by virtue of enbedded structural motives. These features can confer regulatory activity to artificial AS/S pairs such as GFP.
We have developed a technological platform that allows the filtering of �genic� open reading frames from either genomic DNA samples or cDNAs. The availability of ORF libraries, obtained with our filtering method, combined with screening methods such as phage display and protein-protein interaction studies, or with protein structure determination projects, can lead to the identification and structural determination of functional genic ORFs (domainome).
The project's aim is to exploit such a platform to profile the RNA binding domainome, in particular of AS lncRNAs.