Gianni Dehò
Gianni Dehò
affiliation: Università di Milano
research area(s): Genetics And Genomics, Molecular Biology
Course: Biomolecular Sciences
University/Istitution: Università di Milano
Gianni Dehò is Professor of General Microbiology at the University of Milan, where has been teaching Molecular Genetics, Microbial Genetics, and Microbiology, since 1983.
Has been doing research at: Department of Genetics, University of Milan; Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston; Department of Molecular Biology, University of California, Berkeley; Institutionen for Mikrobiologisk Genetik, Karolinska Institutet, Stockholm.
Gianni Dehò has been working in the field of molecular bacterial Genetics since 1969. His main research interests concern the study of gene expression control in prokaryotes. The model system used has been mainly, for several years, the satellite bacteriophage-plasmid P4 and its host Escherichia coli. In particular, in collaboration with Prof. D. Ghisotti, University of Milan, he has characterized a regulatory system at the level of transcription termination controlled by a small antisense RNA. These studies provided a link to more recent research interests focused on RNA maturation and turnover in E. coli and post-transcriptional regulatory mechanisms operating during cold adaptation.
More recently, in collaboration with Prof. A. Polissi, University of Milano-Bicocca, he has been interested in genome-wide approaches for the identification of new essential functions in E. coli. This research led to studies on a cluster of new essential genes conserved in Gram-negative bacteria implicated in outer membrane biogenesis and in the identification and characterization of three genes responsible for the transport of lipopolysaccharide across the periplasmic space.
- Briani, F., Curti, S., Rossi, F., Carzaniga, T., Mauri, P., and Dehò, G. (2008) Polynucleotide phosphorylase hinders mRNA degradation upon ribosomal protein S1 overexpression in Escherichia coli. RNA 14:1-13.
- Del Favero, M., Mazzantini, E., Briani, F., Zangrossi, S., Tortora, P., and Dehò, G. (2008) Regulation of Escherichia coli polynucleotide phosphorylase by ATP. J. Biol. Chem. 283:27355-27359
- Mauri, P. and Dehò, G. (2008) A proteomic approach to the analysis of RNA degradosome composition in Escherichia coli. Methods Enzymol. 447:99-117.
- Sperandeo P., Lau F., Carpentieri A., De Castro C., Molinaro A., Dehò G., Silhavy T.J., and Polissi A. (2008) Functional analysis of the protein machinery required for transport of lipopolysaccharide to the outer membrane of Escherichia coli. J. Bacteriol. 190:4460–4469
- Suits, M.D.L., Sperandeo, P., Dehò, G., Polissi A., and Jia Z. (2008) Novel structure of the conserved Gram-negative lipopolysaccharide transport protein LptA and mutagenesis analysis. J. Mol. Biol. 380:476-488
- Carzaniga, T., Briani, F., Zangrossi, S., Merlino, G., Marchi, P., and Dehò, G. (2009) Autogenous regulation of Escherichia coli polynucleotide phosphorylase expression revisited. J Bacteriol. 191:1738–1748
- Sperandeo, P., Dehò, G., and Polissi, A. (2009) The Lipopolysaccharide transport system of Gram-negative bacteria. Biochim. Biophys.Acta 1791:594–602.
- Gourlay, L. J., Sommaruga, S., Nardini M., Sperandeo, P., Dehò, G., Polissi, A., Bolognesi, M. (2010). Probing the active site of the sugar isomerase domain from E. coli arabinose-5-phosphate isomerase via X-ray crystallography. Protein Sci. 19:2430-2439.
- Delvillani, F. Papiani, Dehò, G., and Briani, F. (2011) S1 ribosomal protein and the interplay between translation and mRNA decay. Nucleic Acids Res.; doi: 10.1093/nar/gkr417.
- Martorana, A.M., Sperandeo, P., Polissi, A., and Dehò, G. (2011) A complex transcriptional organization regulates an Escherichia coli locus implicated in lipopolysaccharide biogenesis. Res. Microbiol. 162:470-482
- Sperandeo, P., Villa, R., Martorana, A.M., Šamalikova, M., Grandori, R., Dehò, G., and Polissi, A. (2011) New insights into the Lpt machinery for lipopolysaccharide transport to the cell surface: LptA-LptC interaction and LptA stability as sensors of a properly assembled transenvelope complex. J. Bacteriol. 193:1042-1053
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