Roberto Mantovani
Roberto Mantovani
affiliation: Università di Milano
research area(s): Genetics And Genomics, Molecular Biology
Course: Biomolecular Sciences
University/Istitution: Università di Milano
orn in Cremona (I), married with Cristina Di Toma, two kids, Mathilde (19) and Pietro (17).
1979 Classic Lyceum C.S.Carlo, Milano.
1979-1985 MD degree. U of Milano. 110/110 cum laude.
1985-1989. PhD Biochemistry. Lab of S. Ottolenghi. U di Milano.
1989-1992. Post-doc fellow. LGME Strasbourg (F) Lab of C. Benoist, D. Mathis. EMBO and EU fellow.
1992-1998. Assistant Professor, Dipartimento di Genetica, U. di Milano.
1998-2002. Associate Professor. Dipartimento di Biologia Animale. U. di Modena (I).
2003-2005. Associate Professor. Dipartimento di Scienze Biomolecolari e Biotecnologie. U. di Milano.
2005- Professor. Genetics. Dipartimento di Scienze Biomolecolari e Biotecnologie. U. di Milano.
The Mantovani lab started in 1993, after he was appointed as Assistant Professor at the U of Milano. His major focus has been on dissection of the molecular mechanisms leading to transcriptional regulation. In particular the lab has focused on three topics.
1) Structure-function of the CCAAT-binding factor NF-Y. The lab has set-up numerous in vitro and in vivo systems and collaborated in crystallization of the trimer. It has performed ChIP on chip studies to identify genomic targets; it is involved in the ENCODE project. The lab has published >65 papers on NF-Y since 1994.
2) Identification of genomic targets of p63, a p53 homologue that is crucial in the development and maintenance of multi-layered epithelia. The lab has performed ChIP on Chip experiments and is reconstructing the network of interactions between p63 and coregulated TFs in human keratinocytes. The lab has published > 15 papers on p63 since 2002.
3) Studies on small compounds that interfere with transcriptional regulation. The lab focused on the mechanisms of action of Et-743. The lab has published > 13 papers on this topic.
• Testoni B. and Mantovani R. Nucleic Acids Res. 34, 928-938 (2006).
• Donati G., Imbriano C. and Mantovani R. Nucleic Acids Res. 34, 3116-27. (2006).
• Viganò MA., Lamartine J., Testoni B., Merico D., Alotto D., Castagnoli C., Robert A., Candi E., Melino G., Gidrol X., and Mantovani R. EMBO J. 25, 5105-16. (2006).
• Viganò M.A. and Mantovani R. Cell Cycle 6, 233-9 (2007).
• Hochhauser D., Kotecha M., O’Hare C., Hartley JM., Taherbhai T., Harris D., Forni C., Mantovani R., Lee M. and Hartley J.A. Mol. Cancer Therapeutics 6, 346-54 (2007).
• Candi E., Rufini A., Terrinoni A., Giamboi-Miraglia A., Lena A-M., Mantovani R., Knight R. and Melino G. Proc. Natl. Acad. Sci USA 104, 11999-12004 (2007).
• D'Incalci M., Brunelli D., Marangon E., Simone M., Tavecchio M., Gescher A., Mantovani R. Curr. Pharm. Des. 13, 2744-50 (2007).
• Kotecha M., Wells G. O’Hare C., Mantovani R., Howard PW. Morris P., Thurston DE., Hartley JA. and Hochhauser D. Mol. Cancer Therapeutics 7, 1319-28 (2008).
• Ceribelli M., Dolfini D., Merico D., Gatta R., Viganò M.A., Pavesi, G. and Mantovani R. Mol. Cell. Biol. 28, 2047-2058 (2008).
• Borrelli S., Candi E., Alotto D., Castagnoli C., Melino G., Viganò MA. and Mantovani R. Cell Death and Differentiation. 16, 253-263 (2008).
• Donati G., Gatta R., Dolfini D., Fossati A., Ceribelli M. and Mantovani R. PLoS ONE, 3(4):e2066 (2008)
• Manni I., Caretti G., Artuso S., Emiliozzi V., Sacchi A., Mantovani R., and Piaggio R. Mol. Biol. Cell. 19, 5203-5213 (2008)
• Göransson M., Andersson MK., Forni C., Ståhlberg A., Andersson C., Olofsson A., Mantovani R. and Åman P. Oncogene 28, 270-278 (2008).
• Gatta R. and Mantovani R. Nucleic Acids Res. 36, 6592-6607 (2008).
• Forni C., Minuzzo M., Virdis M., Tamborini E., Simone M., Tavecchio M., Erba E., Grosso F., Gronchi A., Aman P., Casali P., D’Incalci M., Pilotti S. and Mantovani R. Mol. Cancer Therapeutics 8, 449-457 (2009).
• Aberdam D., Mantovani R. Cell Death and Differentiation. 16, 1073-74 (2009)
• Ceribelli M, Benatti P, Imbriano C, and Mantovani R. J. Biol. Chem. 284, 34189-34200. (2009)
• Dolfini D., Zambelli F., Pavesi G., Mantovani R. Cell Cycle, 8, 4127-4237 (2009)
• Pozzi S., Zambelli F., Merico D., Pavesi G.,Gidrol X., Mantovani R.* and Viganò MA.* PLoS ONE e5008 (2009).
• Pozzi S., Boergesen M., Mandrup S and Mantovani R. J. Invest. Dermatology 129, 2376-85 (2009).
• Gatta R. and Mantovani R. Cell Cycle, 9, 1-11 (2010)
• Borrelli S., Candi E., Hu B., Ravo M., Viganò MA., Weisz A., Dotto GP., Melino G. and Mantovani R. Cell Death and Differentiation, 17, 1896-907 (2010).
• Borrelli S., Fanoni D., Dolfini D., Alotto D., Ravo M., Grober OM., Weisz A., Castagnoli C., Berti E., Vigano AM., Mantovani R. PLoS ONE 5, e13789 (2010).
• Cordani N., Pozzi S., Martynova E., Fanoni D., Borrelli S., Alotto D, Castagnoli C., Berti E., Viganò MA, Mantovani R. Oncogene, in press (2010).
• Hughes R., Kristiansen1 M., Lassot I., Dessagher S., Mantovani R and Ham J. Cell Death and Differentiation, in press (2011).
• Fossati A., Dolfini D., Donati G. Mantovani R. PLoS ONE 6:e17220 (2011).
• Gatta R. and Mantovani R. Epigenetics 6, 526-34 (2011).
• Browne G., Cipollone R., Lena AM., Zhou JH., van Bokhoven H., Doetsch V., Mantovani R. Terrinoni A., Knight RA., Candi E and Melino G. J. Cell Science 124, 2200-7 (2011).
• Gatta R., Dolfini D., Zambelli F., Imbriano C., Pavesi G. and Mantovani R. Epigenetics in press (2011).
• Castel D., Debily M-A., Wu N., Viganò MA., Frouin V., Alibert O., Mantovani R., Romeo P-H Gidrol X. J. Biol. Chem. 286, 20870-9 (2011).
• Gatta R., Dolfini D. and Mantovani R. Cell Death and Disease, 2, e162 (2011).
Project Title:
Determination of the NF-Y “code”.
NF-Y, a complex composed of three subunits, binds to the CCAAT box, one of the most frequent and crucial eukaryotic promoter elements. The structure of NF-Y is related to H2A-H2B, two core histones. Histones are abundant nuclear proteins that wrap around DNA, to compact it in the fundamental unit of chromatin, the nucleosome. Histones are post-translationally modified -acetylation, methylation, ubiquitination- resulting in “code” that specifies specific chromosomal territories. In correlative genome-wide studies, NF-Y overlapped significantly with crucial “positive” histone PTMs, H3K4me3 and H3K9-14ac, correlating with gene expression. Importantly, the impact of NF-Y removal was dramatic on many histones methylations and acetylations, suggesting that NF-Y is at the heart of the regulation of histone PTMs in core promoters. NF-Y is also modified post-translationally, through at least three modifications, acetylation (NF-YA and NF-YB), phosphorylation (NF-YA) and ubiquitination (NF-YB). The Project will focus on finding what are the NF-Y PTMs on each subunits, identifying the relevant aminoacids and determining the role of the most abundant NF-Y PTMs in transcription, and, more generally, in DNA metabolism.