Daniela Ghisotti
Daniela Ghisotti
affiliation: Dept of Biomolecular Science and Biotechnology
research area(s): Genetics And Genomics, Molecular Biology
Course: Biomolecular Sciences
University/Istitution: Università di Milano
Prof. Daniela Ghisotti
Born May 8th, 1950, Milano. Married.
Home address: Via Piero Capponi 4, 20145 Milano.

1974 - Doctoral Degree in Biological Sciences (University of Milano)

Department of Biomolecular Sciences and Biotechnology
University of Milano
Via Celoria 26, 20133 Milano
tel. +39-02-50315020
Fax +39-02-50315044
E-mail: daniela.ghisotti@unimi.it

1975-1978: fellowship from the University of Milano
1978-1985: Research assistant in Genetics
From 1985: Associated Professor of Genetics at the Department of Biomolecular Sciences and Biotechnology (University of Milano)

She is author of 41 publications in peer reviewed international journals, and 3 chapters in international books.

She has obtained several grants from different sources. The more recent are: EC-VI framework NM4TB 2005-2010, EC-VI framework Mycomancy 2006-2009, PUR 2009.

- Prof. Giovanni Dehò - Dipartimento di Scienze Biomolecolari e Biotecnologie - University of Milano (Italy)
- Prof. Giovanna Riccardi - Dipartimento di Genetica e Microbiologia "A. Buzzati-Traverso" - University of Pavia (Italy)
- Prof. Riccardo Manganelli - Dipartimento di Istologia, Microbiologia e Biotecnologie mediche - University of Padova (Italy)
- Dott.ssa Federica Briani - Dipartimento di Scienze Biomolecolari e Biotecnologie - University of Milano (Italy)
- Dr. Harald Putzer - CNRS UPR 9073 - Institut de Biologie Physico-Chimique - Paris (France)
- Dr. Daniela Cirillo - DIBIT S.Raffaele - Milano (Italy).

She has been occasionally a referee for the following journals: Molecular Microbiology, FEMS Microbiology Letters, Research in Microbiology, Journal of Bacteriology, Journal of Molecular Biology, Nucleic Acids Research, Journal of Virology, Virology, Annals of Microbiology, BioTechniques.
She has principally worked in the fields of bacterial genetics and transcriptional regulation of gene expression. She previously worked on Escherichia coli and its phages, in collaboration with Prof. Dehò’s group, and discovered and characterized one of the first phage-encoded small RNAs involved in the control of transcription termination.
From 1997 she started to study Mycobacterium tuberculosis and Mycobacterium smegmatis oxidative stress response. She identified the furA-katG transcripts, a regulated promoter, and an RNase E processing site. Microarray analysis of the furA transcriptome is in progress.
She participated to the NM4TB project, constructing an inducible mycobacterial promoter system, used to create conditional mutants in essential Mtb genes. Several new essential genes were isolated and are under characterization.
She also partecipated to the Mycomancy project, for identification of genes involved in cell cycle regulation.
Now she is involved in a project on identification and characterization of small RNAs in Mtb in collaboration with Dr. D. Cirillo (DIBIT - S.Raffaele - Milan).
Forti F, Mauri V, Deho' G, Ghisotti D. 2011. Isolation of Conditional Expression Mutants in Mycobacterium tuberculosis by Transposon Mutagenesis. Tuberculosis, in press.

Forti F, Crosta A, Ghisotti D. 2009. Pristinamycin-inducible gene regulation in mycobacteria. J Biotechnol. 140:270-7.

Sala C, Forti F, Magnoni F, Ghisotti D. 2008. The katG mRNA of Mycobacterium tuberculosis and Mycobacterium smegmatis is processed at its 5' end and is stabilized by both a polypurine sequence and translation initiation. BMC Mol Biol. 9:33.

Terzano S, Oliva I, Forti F, Sala C, Magnoni F, Dehò G, Ghisotti D. 2007. Bacteriophage P4 sut1: a mutation suppressing transcription termination. J Gen Virol. 88:1041-7.

Magnoni F, Sala C, Forti F, Dehò G, Ghisotti D. 2006. DNA replication in phage P4: characterization of replicon II. Plasmid. 56:216-22.

Piazzolla D, Calì S, Spoldi E, Forti F, Sala C, Magnoni F, Dehò G, Ghisotti D. 2006. Expression of phage P4 integrase is regulated negatively by both Int and Vis. J Gen Virol. 87:2423-31.
No projects are available to students for the current accademic year.