Chiara Mondello
Chiara Mondello
e-mail:
affiliation: Istituto di Genetica Molecolare-CNR, Pavia
research area(s): Cancer Biology, Molecular Biology
Course: Genetics, Molecular and Cellular Biology
University/Istitution: Università di Pavia
Education
1980: Graduation in Biological Sciences cum laude, University of Pavia, Italy.
1984: Advanced Studies in Genetics cum laude, University of Pavia, Italy.
1987: PhD in Genetics and Molecular Biology, University of Pavia, Italy.

Professional positions
1981: Fellowship of the University of Pavia, Istituto di Genetica Biochimica ed Evoluzionistica of the Consiglio Nazionale delle Ricerche (CNR).
1982-1983: CNR Fellowship, Istituto di Genetica Biochimica ed Evoluzionistica, CNR, Pavia (Italy).
1984-1985: Post-doctoral EMBO Fellowship, Imperial Cancer research Fund, London (UK).
1986-2001: CNR Researcher, Istituto di Genetica Biochimica ed Evoluzionistica, CNR, Pavia, Italy.
1992-1994; 2011-present: Professor a contratto, University of Pavia (Faculty of Science).
2001-present: Senior Researcher, Istituto di Genetica Molecolare of CNR, Pavia, Italy.
2002-2009: Member of the Scientific Committee of the Istituto di Genetica Molecolare of CNR.
2005-present: Member of the CNR list of: "Esperti in gestione progettuale".

Memberships
Member of the New York Academy of Science.
Member of the Italian Genetic Association.

Editorial activity
Member of the Editorial Board of "Stem Cell Discovery", "ISRN Genomics" and "ScienceJet Journal"

Leadership in projects
1995-1997: AIRC project: Genome Instability in XP fibroblasts used as a model to study cellular transformation».
1996-1998: Partner in the EU BIOMED 2 Concerted Action: «Mammalian chromosome stability and cancer».
2001-2003: Partner in the EU project “Telomere instability and the formation and transmission of radiation induced DNA damage”.
2003-2005: Partner in the EU project “Telomeres and radiosensitivity of individuals”
2004-2008: FIRB Project from Italian Ministry of Research “Nuovi sistemi cellulari per studiare la riparazione delle rotture a doppio filamento del DNA in cellule di mammifero”.
2007-2010: Project funded by Fondazione Cariplo “Genomics of tumor progression using a model cellular system.”
2011-2013: Partner in the project funded by Fondazione Cariplo “Optofluidic chips for the study of cancer cell mechanical properties and invasive capacities.”

Reviewer activity
Reviewer for the following scientific journals: European Journal of Cancer; Proc. Nat. Acad. Science USA; BMC Medical Genetics; Mechanisms of Ageing and Development; Biochimie; Cytogenetics and Genome Research; Molecular Cancer Research; Carcinogenesis; Mutation Research Rewiew chapters in Springer books.
Evaluator of scientific projects for Swiss Cancer League, Swiss National Science Foundation, Belgian FNRS (Fonds de la Recherche Scientifique), Singapore National Medical Research Council.
Genome instability is a hallmark of cancer cells. The acquisition of successive genomic alterations through several different mechanisms is the engine of neoplastic transformation. The interest of our laboratory is focused on the stepwise process of transformation and on mechanisms causing genomic instability. We have developed a telomerase human fibroblast cellular system in which restoration of telomerase activity has been followed not only by immortalization, but also by the acquisition of the neoplastic phenotype. During culture propagation, immortalized cells became more malignant, as shown by a decreased latency in tumor development when injected in immunocompromised mice. By comparing cells at different stages of propagation, we have managed to identify some cellular and molecular alterations specifically associated with different phases of transformation. We are currently interested in exploiting this cellular system to deepen our knowledge on the genomic modifications accompanying cellular transformation. In particular, by comparing global gene and miRNA expression profiles in cells with different degrees of malignancy, we are investigating molecular changes involved in the acquisition of the metastatic phenotype.

1. Rebuzzini P., Martinelli P., Blasco M., Giulotto E. and Mondello C. Inhibition of gene amplification in telomerase deficient immortalized mouse embryonic fibroblasts. Carcinogenesis 283: 553-559 (2007). Epub 2006 Sep 14.
2. Paulis M.,. Bensi M., Orioli D., Mondello C., Mazzini G., D'Incalci M., Falcioni C., Radaelli E., Erba E., Raimondi E., and De Carli L. Transfer of a human chromosomal vector from a hamster cell line to a mouse embryonic stem cell line. Stem Cells 25: 2543-2550 (2007). Epub 2007 Jul 5.
3. Nergadze S.G., Santagostino M.A., Salzano A., Mondello C., and Giulotto E. Contribution of telomerase RNA retrotranscription to DNA double-strand break repair during mammalian genome evolution. Genome Biol. 8: R260 (2007)
4. Donà F., Mondello C. and Scovassi A.I. Poly (ADP-ribosylation) at telomeres. Trends in Cell & Molecular Biology 2: 77-88 (2007).
5. Zongaro S., Verri A., Giulotto E., and Mondello C. Telomere length and radiosensitivity in human fibroblast clones immortalized by ectopic telomerase expression. Oncology Reports 19: 1605-1609 (2008).
6. Donà F., Prosperi E., Savio M., Coppa T., Scovassi A. I., and Mondello C. Loss of Histone H2AX increases sensitivity of immortalized mouse fibroblasts to the topoisomerase inhibitor etoposide. Int. J. Oncol. 33: 613-621 (2008).
7. Belgiovine C., Chiodi I., and Mondello C. Telomerase: cellular immortalization and neoplastic transformation. Multiple functions of a multifaceted complex. Cytogenetic and Genome Research. 122: 255-262 (2008).
8. Maga G., Crespan E., Wimmer U., van Loon B., Amoroso A., Mondello C., Belgiovine C., Ferrari E., Villani G. and Hübscher U. Replication Protein A and Proliferating Cell Nuclear Antigen coordinate DNA polymerase selection in 8-oxo-guanine repair. Proc. Natl. Acad. Sci. USA. 105: 20689-20694 (2008).
9. Salzano A., Kochiashvili N., Nergadze S.G., Khoriauli L., Smirnova A., Herrera A., Mondello C., and Giulotto E. Enhanced gene amplification in human cells knocked down for DNA-PKcs. DNA Repair 8: 19-28 (2009).
10. Mondello C., Smirnova A., and Giulotto E. Gene amplification, radiation sensitivity and DNA double strand breaks. Mutat. Res. 704: 29-37 (2010). Epub ahead of print January 2010.
11. Belgiovine C., Frapolli R., Bonezzi K., Chiodi I., Mello-Grand M., Favero F., Dei Tos A. P., Giulotto E., Taraboletti G., D’Incalci M., Mondello C. ROCK inhibitor RhoE is associated with increased invasiveness and metastatic potential in mesenchymal tumor cells. PLoS ONE 5: e141542010 (2010).
12. Mondello C. and Scovassi I. Apoptosis: a way to maintain healthy individuals. In “Genome Stability and Human Diseases” Nasheuer, H.P. (Ed.). Subcell Biochem. 50: 307-323 (2010). ISBN 978-90-481-3470-0
13. Ruiz-Herrera A., Smirnova A., Khouriauli L., Nergadze S.G., Mondello C. and Giulotto E. Gene amplification in human cells knocked down for RAD54. Genome Integrity. 2: 5 (2011).
14. Chiodi I., Belgiovine C., Donà I., Scovassi A.I. and Mondello C. Drug treatment of cancer cell line: a way to select for cancer stem cells? Cancers 3: 1111-1128 (2011) doi:10.3390/cancers3011111.
15. Belgiovine C., Chiodi I. and Mondello C. Relocalization of cell adhesion molecules during neoplastic transformation of human fibroblasts. Int. J. Oncol. 39: 1199-1204. 2011.
16. Ostano P., Bione S., Belgiovine C., Chiodi I., Ghmenti C., Scovassi A.I., Chiorino G. and Mondello C. Cross-analysis of gene and miRNA genome-wide expression profiles in human fibroblasts at different stages of transformation. OMICS 16: 24-36, 2012.
17. Donà F., Chiodi I., Belgiovine C., Raineri T., Ricotti R., Mondello C. and Scovassi A.I. Poly(ADP-ribosylation) and neoplastic transformation: effect of PARP inhibitors. Current Pharm. Biotechnol. 2012 Mar 20. [Epub ahead of print].
18. Vidale P., Magnani E., Nergadze S.G., Santagostino M., Cristofari G., Smirnova A., Mondello C. and Giulotto E. The catalytic and the RNA subunits of human telomerase are required to immortalize equid primary fibroblasts. Chromosoma 121: 475-488, 2012. Epub 2012 Jul 14.
19. Bragheri F., Minzioni P., Martinez Vazquez R., Bellini N., Paiè P., Mondello C., Ramponi R., Cristiani I. and Osellame R. Optofluidic integrated cell sorter fabricated by femtosecond lasers. Lab Chip 12: 3779-384, 2012.
20. Chiodi I. and Mondello C. Telomere-independent functions of telomerase in nuclei, cytoplasm, and mitochondria. Frontiers in Oncology “Telomeres: structure, functions and therapeutic opportunities”. Front Oncol. 2:133, 2012. doi: 10.3389/fonc.2012.00133. Epub 2012 Sep 28.
21. Chiodi I., Belgiovine C., Zongaro S., Ricotti R., Horard B., Lossani A., Focher F., Gilson E., Giulotto E. and Mondello C. Super-telomeres in transformed human fibroblasts. Biochim Biophys Acta. 1833:1885-1893, 2013. Epub 2013 Apr 6. doi: 10.1016/j.bbamcr.2013.03.030.

No projects are available to students for the current accademic year.