Donata Orioli
Donata Orioli
affiliation: Istituto di Genetica Molecolare-CNR, Pavia
research area(s): Cell Biology, Neuroscience
Course: Genetics, Molecular and Cellular Biology
University/Istitution: Università di Pavia
1997: Ph.D. with first-class honours in Natural Sciences, University of Heidelberg, Germany.
1991: Graduated cum laude in Biological Sciences, University of Pavia.

2009-at present: CNR Researcher, IGM CNR Pavia.
2008: Visiting scientist at the Institute of Genetics and Molecular and Cellular Biology of Strasbourg, France.
2006-2008: Research fellow supported by Cariplo Foundation, Italian Association for Cancer Research (AIRC) and European Community, IGM CNR Pavia.
2003-2006: Research contractor supported by the FIRB grant RBNE01RNN7, IGM CNR of Pavia.
2000-2002: Principal investigator of a research project sponsored by Telethon Foundation at the IGM CNR Pavia.
2000: EMBO Post-Doctoral Fellow, IGM CNR of Pavia.
1998: EMBO Post-Doctoral Fellow, DIBIT-San Raffaele Hospital, Milano.
1993-1997: PhD fellow, European Molecular Biology Laboratory (EMBL), laboratory of Rüdiger Klein, Heidelberg-Germany
1993: Erasmus fellow, EMBL, laboratory of Marino Zerial, Heidelberg-Germany.

2009-2011: Supervisor of the research project "Trichothiodystrophy as a model disease to dissect the basis of TFIIH transcriptional activity", sponsored by CNR-CNRS international agreement.
Since 2003 member of a research unit participating to research projects sponsored by the Italian Ministry of Education, University and Research (MIUR), by the European Community, by Cariplo Foundation and by the Italian Association for Cancer Research (AIRC).
2000-2002: Principal Investigator of the research project "Identification of Rac3-interacting proteins during neuritogenesis", sponsored by the Italian Telethon Foundation (contract 459/bi).
Invited speaker at several national and international meetings and workshops.

since 2010: In charge of the course Molecular Genetics, Laurea Magistralis in Molecular Biology and Genetics, University of Pavia.
2005-2008: In charge of the course Ingegneria Genetica (BIO/11- Biologia Molecolare), University of Pavia.
1998-1999: In charge of the course "Regolazione molecolare delle proiezioni delle cellule nervose dell'Embrione", University of Bologna.
Extensive experience and publication record in the field of molecular biology and biochemistry, with special emphasis on nervous system development in mammals. In the last years, her research has been focusing on the human syndromes xeroderma pigmentosum (XP), trichothiodistrophy (TTD) and Cockayne Syndrome (CS), which result from defects in nucleotide excision repair (NER), a versatile system that removes a wide range of lesions, including UV photoproducts. By microarray analysis, she has recently identified genes specifically deregulated in primary skin fibroblasts derived from TTD patients. The analysis has allowed to identify alterations in the extracellular matrix of TTD cells that could explain some of TTD clinical symptoms and to define new functions of the repair/transcription complex TFIIH in transcription.
Murphy C, Orioli D, Lutcke A, Bucci C, Chiariello M, Lehtonen E and Zerial M. (1994) Rab proteins and the regulation of vesicular traffic in polarised cells. In: GTPase-controlled molecular machines. Challenges of Modern Medicine. Corda D, Hamm H, Luini A eds. pp 187-195.
Gassmann M, Casagranda F, Orioli D, Simon H, Lai C, Klein R, and Lemke G. (1995) Aberrant neuronal and cardiac development in mice lacking the ErbB4 neuregulin receptor. Nature 378: 390-394.
Peverali FA, Orioli D, Tonon L, Ciana P, Bunone G, Negri M and Della Valle G. (1996) Retinoic acid-induced growth arrest and differentiation of neuroblastoma cells are counteracted by N-myc and enhanced by max overexpression. Oncogene 12: 457-462.
Henkemeyer M*, Orioli D*, Henderson JT, Saxton TM, Roder J, Pawson T and Klein R. (1996) Nuk controls pathfinding of commissural axons in the mammalian central nervous system. Cell 86: 35-46.
*The first two authors equally contributed to this article
Orioli D*, Henkemeyer M*, Lemke G, Klein R and Pawson T. (1996) Sek4 and Nuk receptors cooperate in guidance of commissural axons and in palate formation. EMBO J. 15: 6035-6049.
*The first two authors equally contributed to this article
Brambilla R, Bruckner K, Orioli D, Bergemann AD, Flanagan JG and Klein R. (1996) Similarities and differences in the way transmembrane-type ligands interact with the Elk subclass of Eph receptors. Mol. Cell. Neurosc. 8: 199-209.
Orioli D and Klein R. (1997) The Eph receptor family: axonal guidance by contact repulsion. Trends in Genetics 13: 354-359.
Anderson DJ, Barbacid M, Berg LJ, Bergemann AD, Bonhoeffer F, Bohme B, Boyd AW, Brandli AW, Bronner-Fraser M, Caras IW, Cerretti DP, Chambon P, Charnay P, Cheng HJ, Ciossek T, Daar IO, Davis S, Dixit VM, Drescher U, Faissner A, Flanagan JG, Fletcher FA, Fox GM, Frisen J, Gale NW, Gilardi-Hebenstreit P, Goodman CS, Hemmati-Brivanlou A, Henkemeyer M, Hirai H, Holder N, Holland SJ, Hunter T, Ikegaki N, Klein R, Koblar SA, Krull CE, Lansford R, Lemke G, Lindberg RA, Lyman SD, Maisonpierre PC, Marcelle C, Miescher GC, Monschau B, Nicola NA, Nieto MA, Ohta K, O"Leary DDM, Orioli D, Pasquale EB, Pawson T, Reith AD , Rogers J H , Rohrer B M , Sanes J R , Sargent TD , Scales JB , Schindelholz B, Siever DA, Strebhardt K, Sugimura H, Tanaka H, Tang XX, Tessier-Lavigne M, Ullrich A, Valenzuela DM, Verderame MF, Wanaka A, Watt VM, Welcher AA, Wilkinson DG, Winning RS, Yancopoulos GD, Zhou R, Ziemiecki A, Zipursky SL. (1997) Unified Nomenclature for Eph Family Receptors and Their Ligands, The Ephrins. Cell 90: 403-404.
Zacchi P, Stenmark H, Parton RG, Orioli D, Lim F, Giner A, Mellman I, Zerial M and Murphy C. (1998) Rab17 regulates membrane trafficking through apical recycling endosomes in polarized epithelial cells. J. Cell Biol. 140: 1039-1053.
Orioli D*, Colaluca IN, Stefanini M, Riva S, Dotti GC and Peverali AF. (2006) Rac3-induced neuritogenesis requires the binding to Neurabin I. Molecular Biology of the Cell 17: 2391-2400.
* corresponding author
D"Errico M, Parlanti E, Teson M, Degan P, Lemma T, Calcagnile A, Iavarone I, Jaruga P, Ropolo M, Pedrini AM, Orioli D, Frosina G, Zambruno G, Dizdaroglu M, Stefanini M and Dogliotti E. (2007) The role of CSA in the response to oxidative DNA damage in human cells. Oncogene 26 (30): 4336-4343.
Paulis M, Bensi M, Orioli D, Mazzini G, D"Incalci M, Falcioni C, Radaelli E, Mondello C, Raimondi E and De Carli L. (2007) Transfer of a human chromosomal vector from a hamster cell line to a mouse embryonic stem cell line. Stem Cell, 10: 2543-2550
Yasuda G, Watanabe E, Nishi R, Mori T, Iwai S, Orioli D, Stefanini M, Hanaoka F and Sugasawa K (2007) In vivo instability and functional defects of XPC protein caused by a pathogenic missense mutation. Mol Cell Biol. 19: 6606-6614.
Botta E, Nardo T, Orioli D, Guglielmino R, Ricotti R, Bondanza S, Benedicenti F, Zambruno G, Stefanini M. (2009) Genotype-phenotype relationships in trichothiodystrophy patients with novel splicing mutations in the XPD gene. Hum Mutat. 30:438-45.
Nardo T, Oneda R, Spivak G, Vaz B, Mortier L, Thomas P, Orioli D, Laugel V, Stary A, Hanawalt PC, Sarasin A, Stefanini M. (2009) A UV-sensitive syndrome patient with a specific CSA mutation reveals separable roles for CSA in response to UV and oxidative DNA damage. Proc Natl Acad Sci USA. 106:6209-14.
Stefanini M, Botta E, Lanzafame M and Orioli D. (2010) Trichothiodystrophy: from basic mechanisms to clinical implications. DNA Repair. 9:2-10.
Project Title:
Trichothiodystrophy as a model disease to dissect the basis of TFIIH activity in transcription, DNA repair and cell cycle progression

Project Title:
Roles of the genes responsible for Cockayne syndrome in ageing and neurodegeneration