Eugenio Notomista
Eugenio Notomista
affiliation: Università di Napoli Federico II
research area(s): Computational Biology
Course: Computational Biology and Bioinformatics
University/Istitution: Università di Napoli Federico II
2000: PhD in Biological Chemistry and Molecular Biology. Title of the thesis: “Una ribonucleasi antitumorale: l’onconasi. Proprietà chimico-fisiche e biologiche”
2001: Post Doctoral fellowship at the institute IRBM-P. Angeletti SPA.
2002-2003: “Assegno Di Collaborazione Ad Attivita' Di Ricerca Ex-Art.51”, at the Dept of Biological Chemistry-University of Naples Federico II.
2004 to date: Researcher, Faculty of Biotechnological Sciences, University of Naples Federico II.
Teaching activity
a.y. 2004/05: “Professore supplente” for the course “Laboratorio di Biochimica”, Faculty of Biotechnological Sciences, University of Naples Federico II.
a.y. 2004/05 and 2005/06: “Professore supplente” for the course of Biochemistry, “Scuola di Specializzazione in Fisica Sanitaria”, University of Naples Federico II.

a.y. 2004/05 to date: “Professore aggregato” for the course of Bioinformatics, Faculty of Biotechnological Sciences, University of Naples Federico II.
Dr. Notomista has focused his research themes on the structure-to-function relationships in proteins, protein evolution and protein structure analysis, protein modelling, small ligand docking and characterization of protein/membrane and peptide/membrane interactions.
Some representative scientific research themes are:
- Structure and enzymology of ribonucleases from vertebrates.
- Production of ribonucleases with pharmacological potential by means of protein engineering.
- Structure and enzymology of monooxygenases, aromatic dioxygenases, ring cleavage dioxygenases, laccases and their use in the bioremediation of aromatic compounds
- Evolution of enzymes and metabolic pathways of bacterial strains able to grow on xenobiotics.
- Isolation and characterization of bacterial strains for bioremediations.
- Analysis of bacterial genomes.
- Modelling of the interaction of cationic antimicrobial peptides with membranes, detergent micelles and solid surfaces.
Dr. Notomista’s scientific activity is documented by 23 publications on international journals, 2 patents and 26 communications at National and International Congresses and Meetings.

1- Notomista, E., Pennacchio, F., Cafaro, V., Smaldone, G., Izzo, V., Troncone, L., Varcamonti, M., Di Donato A. (2011) The marine isolate Novosphingobium sp. PP1Y shows specific adaptation to use the aromatic fraction of fuels as the sole carbon and energy source. MICROB ECOL. 61:582-94. Epub 2011 Jan 22.

2- Zanfardino, A., Restaino, O.F., Notomista, E., Cimini, D., Schiraldi, C., De Rosa, M., De Felice, M., & Varcamonti, M. (2010). Isolation of an Escherichia coli K4 kfoC mutant over-producing capsular chondroitin. Microb Cell Fact. 79, 34.

3- Miele, A., Giardina, P., Notomista, E., Piscitelli, A., Sannita, G., & Faraco, V. (2010). A semi-rational approach to engineering laccase enzymes. Mol Biotechnol 46: 149-56.

4- Spadaccini, R., D'Errico, G., D'Alessio, V., Notomista, E., Bianchi, A., Merola, M., & Picone, D. (2010). Structural characterization of the transmembrane proximal region of the hepatitis C virus E1 glycoprotein. Biochim Biophys Acta. 1798:344-53.

5- Notomista, E., Cafaro, V., Bozza, G., Di Donato, A. (2009). The molecular determinants of the regioselectivity of toluene/o-xylene monooxygenase from Pseudomonas sp. OX1. APPLIED AND ENVIRONMENTAL MICROBIOLOGY 75:823- 836.

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